Sukcharoen Oratai, Sirirote Pramote, Thanaboripat Dusanee
Department of Biotechnology, Faculty of Science, Ramkhamhaeng University, Bangkok, 10240 Thailand.
Department of Biology, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok, 10520 Thailand.
J Food Sci Technol. 2017 Aug;54(9):2929-2935. doi: 10.1007/s13197-017-2731-4. Epub 2017 Jun 9.
Essential oil from the bark of (Roxb.) Kosterm was obtained by hydrodistillation using a Clevenger-type system for 3 h and identified by gas chromatography-mass spectrometry. The compounds were safrole (93.9%), elemicine (4.3%) and methyl eugenol (1.7%). The effect of essential oil on mycelial growth, sporulation, and aflatoxin B1 production of IMI 283883 and IMI 242684 was evaluated using contact and vapor treatments. Aflatoxin B1 was determined using the Enzyme-linked immunosorbent assay. The results showed that (Roxb.) Kosterm essential oil at concentrations more than 200 ppm exhibited inhibition effect on mycelial growth, sporulation, and aflatoxin B1 production of both strains as compared with control. The fumigation activities via vapor treatment showed higher inhibition than contact treatment. This study suggests that (Roxb.) Kosterm essential oil represents a good alternative in eco-friendly control of aflatoxigenic strain on food and agricultural commodities.
通过使用克莱文杰型装置进行3小时的水蒸馏，从（罗克斯伯）科斯泰姆树皮中获得精油，并通过气相色谱 - 质谱法进行鉴定。这些化合物为黄樟素（93.9%）、榄香素（4.3%）和甲基丁香酚（1.7%）。使用接触处理和蒸汽处理评估了该精油对黄曲霉IMI 283883和黄曲霉IMI 242684的菌丝生长、孢子形成及黄曲霉毒素B1产生的影响。使用酶联免疫吸附测定法测定黄曲霉毒素B1。结果表明，与对照相比，浓度超过200 ppm的（罗克斯伯）科斯泰姆精油对两种菌株的菌丝生长、孢子形成及黄曲霉毒素B1产生均表现出抑制作用。通过蒸汽处理的熏蒸活性显示出比接触处理更高的抑制作用。本研究表明，（罗克斯伯）科斯泰姆精油是对食品和农产品上产黄曲霉毒素菌株进行生态友好控制的良好替代品。
