Kasrati Ayoub, Alaoui Jamali Chaima, Bekkouche Khalid, Wohlmuth Hans, Leach David, Abbad Abdelaziz
Laboratory of Biotechnology, Protection and Valorization of Plant Resources, Faculty of Science Semlalia, Cadi Ayyad University, B.P. 2390, 40000 Marrakech, Morocco (URAC 35).
Southern Cross Plant Science, Southern Cross University, Lismore, NSW 2480 Australia ; Integria Healthcare, Gallans Rd, Ballina, NSW 2478 Australia.
J Food Sci Technol. 2015 Apr;52(4):2312-9. doi: 10.1007/s13197-014-1284-z. Epub 2014 Feb 16.
This study describes the antioxidant and insecticidal activities of essential oils (EOs) of Mentha suaveolens subsp. timija, Thymus satureioides, Achillea ageratum, Cotula cinerea and Salvia officinalis widely used in Morocco as flavorings, food additives and preservatives. Sixty seven components were identified accounting for more than 95.0 % of the total oils. M. suaveolens subsp. timija oil had as main components menthone and pulegone. A. ageratum oil was particularly rich in artemisyl acetate and yomogi alcohol. The essential oil of T. satureioides was characterized by high contents of carvacrol and borneol. C. cinerea oil contained trans-thujone and cis-verbenyl acetate as major constituents, whereas S. officinalis oil was characterized by trans-thujone and camphor. Antioxidant activities were examined by means of 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), reducing power, β-carotene/linoleic acid bleaching and ABTS radical tests. In all assays, the highest antioxidant potency was observed in T. satureioides EO with IC50 values ranging from 0.15 ± 0.36 μg mL(-1) to 0.23 ± 0.67 μg mL(-1) across the four assays. The in vitro evaluation of the insecticidal activity showed that M. suaveolens subsp. timija EO present the highest insecticidal efficiency against adults of Tribolium castaneum with LD50 and LD90 values of 0.17 μL cm(-2) and 0.26 μL cm(-2), respectively and LT50, LT90 values ranged from 44.19 h to 2.98 h and 98.14 h to 6.02 h, respectively. Our data support the possible use of T. satureioides oil as potential antioxidant agent, while M. suaveolens subsp. timija oil can be developed as a new natural bio-insecticide.
本研究描述了摩洛哥广泛用作调味剂、食品添加剂和防腐剂的柔薄荷亚种蒂米加薄荷、撒丁百里香、香叶蓍、灰香青和药用鼠尾草精油（EOs）的抗氧化和杀虫活性。共鉴定出67种成分，占总油含量的95.0%以上。柔薄荷亚种蒂米加薄荷油的主要成分是薄荷酮和胡薄荷酮。香叶蓍油特别富含乙酸青蒿酯和艾醇。撒丁百里香精油的特征是香芹酚和冰片含量高。灰香青油含有反式侧柏酮和顺式乙酸马鞭草烯酯作为主要成分，而药用鼠尾草精油的特征是反式侧柏酮和樟脑。通过2,2-二苯基-1-苦基肼自由基（DPPH）、还原能力、β-胡萝卜素/亚油酸漂白和ABTS自由基试验检测抗氧化活性。在所有试验中，撒丁百里香精油的抗氧化能力最强，在四项试验中的半数抑制浓度（IC50）值范围为0.15±0.36μg mL-1至0.23±0.67μg mL-1。杀虫活性的体外评估表明，柔薄荷亚种蒂米加薄荷精油对赤拟谷盗成虫的杀虫效率最高，半数致死剂量（LD50）和90%致死剂量（LD90）值分别为0.17μL cm-2和0.26μL cm-2，半数致死时间（LT50）和90%致死时间（LT90）值分别为44.19小时至2.98小时和98.14小时至6.02小时。我们的数据支持撒丁百里香精油可能用作潜在抗氧化剂，而柔薄荷亚种蒂米加薄荷油可开发为一种新型天然生物杀虫剂。
