Henderson Z
Department of Physiology, University College Cardiff, Wales.
J Comp Neurol. 1987 Nov 22;265(4):581-92. doi: 10.1002/cne.902650409.
The distribution of catecholaminergic and cholinergic neurons in the upper brainstem of the ferret were mapped by staining immunohistochemically two adjacent series of sections of brainstem for tyrosine hydroxylase and choline acetyltransferase, respectively. As in other species, large numbers of tyrosine-hydroxylase-positive neurons are localized in the ventral tegmental area (A10), the substantia nigra (A9), and in A8. Tyrosine-hydroxylase-positive neurons in the dorsolateral pontine tegmentum (A4, A6, and A7--the locus coeruleus complex) of the ferret are rather diffusely distributed, as has been observed in other carnivore species such as the cat and the dog, but unlike the cat, these cells in the ferret display a relative uniformity in size and morphology. Choline-acetyltransferase-positive neurons which extend in the ferret's pedunculopontine tegmental nucleus and ventral parabrachial area (Ch5) are relatively large cells that stain intensely for choline acetyltransferase, and their dendrites form prominent bundles in regions where unstained fibre tracts are prevalent. Choline-acetyltransferase-positive neurons distributed in the laterodorsal tegmental nucleus (Ch6) are smaller than the cholinergic cells of Ch5, and they stain less intensely for choline acetyltransferase. Rostrally, there is little overlap between the catecholaminergic cell groups A8, A9, and A10 and the cholinergic cell groups of Ch5 and Ch6. Caudally, the Ch5 neurons extend some considerable extent into the locus coeruleus complex. In the region of overlap, no cells with staining for both tyrosine hydroxylase and choline acetyltransferase were observed, as was ascertained with a double staining method employing a combination of tyrosine hydroxylase immunofluorescence and choline acetyltransferase peroxidase-antiperoxidase immunohistochemistry. In conclusion, the ferret has a typically carnivore pattern for the distribution of catecholaminergic cells in the upper brainstem, and there is a significant overlap between the catecholaminergic and cholinergic cell groups in the dorsolateral pontine tegmentum.
通过分别对脑干相邻的两个系列切片进行免疫组织化学染色，以检测酪氨酸羟化酶和胆碱乙酰转移酶，从而绘制出雪貂上脑干中儿茶酚胺能神经元和胆碱能神经元的分布图。与其他物种一样，大量酪氨酸羟化酶阳性神经元位于腹侧被盖区（A10）、黑质（A9）和A8区。雪貂脑桥背外侧被盖区（A4、A6和A7——蓝斑复合体）中的酪氨酸羟化酶阳性神经元分布较为分散，正如在猫和狗等其他食肉动物物种中所观察到的那样，但与猫不同的是，雪貂中的这些细胞在大小和形态上相对较为一致。延伸至雪貂脚桥被盖核和腹侧臂旁核（Ch5）的胆碱乙酰转移酶阳性神经元是相对较大的细胞，对胆碱乙酰转移酶染色强烈，并且它们的树突在未染色纤维束普遍存在的区域形成明显的束状结构。分布在脑桥背外侧被盖核（Ch6）中的胆碱乙酰转移酶阳性神经元比Ch5的胆碱能细胞小，并且它们对胆碱乙酰转移酶的染色较弱。在头端，儿茶酚胺能细胞群A8、A9和A10与Ch5和Ch6的胆碱能细胞群之间几乎没有重叠。在尾端，Ch5神经元相当程度地延伸至蓝斑复合体。在重叠区域，采用酪氨酸羟化酶免疫荧光和胆碱乙酰转移酶过氧化物酶-抗过氧化物酶免疫组织化学相结合的双重染色方法确定，未观察到同时对酪氨酸羟化酶和胆碱乙酰转移酶染色的细胞。总之，雪貂上脑干中儿茶酚胺能细胞的分布具有典型的食肉动物模式，并且脑桥背外侧被盖区的儿茶酚胺能和胆碱能细胞群之间存在显著重叠。
