Li Diana, Dong Hongli, Kohan Alison B
Department of Nutritional Sciences, University of Connecticut, 1392 Storrs Road, Unit 4010, Storrs, CT, 06269-4010, USA.
Methods Mol Biol. 2019;1576:195-204. doi: 10.1007/7651_2017_69.
Since the initial report in 2009 by Sato and Clevers, primary enteroids have been of major interest in the fields of stem cell biology and gastrointestinal (GI) tract biology. More recently, we and others have made major inroads into the physiological relevance of these enteroid models and have shown that enteroids derived from the stomach, intestine, or colon recapitulate major functions of these tissues, namely, gastric acid secretion, lipid absorption and lipoprotein secretion, and ion transport. Here, we detail the isolation of stem cells from the small intestine and the culture and propagation of those stem cells into mature three-dimensional enteroids. We will also detail how we use enteroids to determine intestinal mechanisms behind dietary lipid absorption and lipoprotein secretion. The primary enteroid model is a powerful tool that significantly expands our ability to model GI tract function in vitro.
自2009年佐藤和克莱弗斯首次发表报告以来，原代肠类细胞一直是干细胞生物学和胃肠道生物学领域的主要研究对象。最近，我们和其他研究人员在这些肠类细胞模型的生理相关性方面取得了重大进展，并表明从胃、小肠或结肠分离得到的肠类细胞能够重现这些组织的主要功能，即胃酸分泌、脂质吸收和脂蛋白分泌以及离子转运。在这里，我们详细介绍从小肠中分离干细胞以及将这些干细胞培养和扩增为成熟的三维肠类细胞的方法。我们还将详细说明如何利用肠类细胞来确定饮食中脂质吸收和脂蛋白分泌背后的肠道机制。原代肠类细胞模型是一个强大的工具，它显著扩展了我们在体外模拟胃肠道功能的能力。
