Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production; Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Changsha, Hunan, 410125, China.
Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, 3015, CE Rotterdam, The Netherlands.
Mol Nutr Food Res. 2019 Aug;63(16):e1801143. doi: 10.1002/mnfr.201801143. Epub 2019 Apr 10.
Nutritional studies are greatly hampered by a paucity of proper models. Previous studies on nutrition have employed conventional cell lines and animal models to gain a better understanding of the field. These models lack certain correlations with human physiological responses, which impede their applications in this field. Enteroids are cultured from intestinal stem cells and include enterocytes, enteroendocrine cells, goblet cells, Paneth cells, and stem cells, which mimic hallmarks of in vivo epithelium and support long-term culture without genetic or physiological changes. Enteroids have been used as models to study the effects of diet and nutrients on intestinal growth and development, ion and nutrient transport, secretory and absorption functions, the intestinal barrier, and location-specific functions of the intestine. In this review, the existing models for nutritional studies are discussed and the importance of enteroids as a new model for nutritional studies is highlighted. Taken together, it is suggested that enteroids can serve as a potential model system to be exploited in nutritional studies.
营养研究受到缺乏适当模型的严重阻碍。以前的营养研究采用传统的细胞系和动物模型来更好地理解该领域。这些模型与人类生理反应缺乏某些相关性，这限制了它们在该领域的应用。类器官是从肠干细胞培养而来的，包括肠上皮细胞、肠内分泌细胞、杯状细胞、潘氏细胞和干细胞，它们模拟体内上皮细胞的特征，并支持长期培养而不发生遗传或生理变化。类器官已被用作研究饮食和营养对肠道生长和发育、离子和营养物质转运、分泌和吸收功能、肠道屏障以及肠道特定部位功能的影响的模型。在这篇综述中，讨论了现有的营养研究模型，并强调了类器官作为营养研究新模型的重要性。总之，建议将类器官作为营养研究中潜在的模型系统加以利用。
