Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture Chinese Academy of Sciences, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture , Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process , Changsha , Hunan 410125 , China.
Department of Gastroenterology and Hepatology , Erasmus MC University Medical Center , 3015 GD Rotterdam , The Netherlands.
J Agric Food Chem. 2019 Mar 6;67(9):2421-2428. doi: 10.1021/acs.jafc.8b06908. Epub 2019 Feb 19.
The lack of sophisticated in vitro models limits our current understanding of gastrointestinal functions in farm animals. Conventional 2D cell lines or primary cells fail to recapitulate the physiology of in vivo intestinal epithelium. In contrast stem cell-derived, nontransformed 3D enteroids partially recreate the villus-crypt anatomy of the native intestine and comprise most if not all intestinal cell types including enterocytes, enteroendocrine cells, goblet cells, Paneth cells, and stem cells. This review summarizes the techniques used for generating and culturing enteroids of various farm animal species, focuses on important factors influencing the longevity of enteroids, and provides an overview of their current applications in modeling veterinary pathogens and in developing chemicals and bioactives for treating animal disease and improving production performance. It also mentions current limitations of enteroid models and potential solutions and highlights the opportunities for using these enteroids as a platform in studies regarding veterinary sciences and animal nutrition.
缺乏复杂的体外模型限制了我们目前对农场动物胃肠道功能的理解。传统的 2D 细胞系或原代细胞无法再现体内肠道上皮的生理学。相比之下，干细胞衍生的、未转化的 3D 类肠上皮细胞部分重现了天然肠道的绒毛-隐窝解剖结构，并且包含了大多数（如果不是全部的话）肠道细胞类型，包括肠上皮细胞、肠内分泌细胞、杯状细胞、潘氏细胞和干细胞。这篇综述总结了用于生成和培养各种农场动物类肠上皮细胞的技术，重点介绍了影响类肠上皮细胞寿命的重要因素，并概述了它们在兽医病原体建模以及开发用于治疗动物疾病和提高生产性能的化学物质和生物活性物质方面的当前应用。它还提到了类肠上皮细胞模型的当前限制和潜在解决方案，并强调了将这些类肠上皮细胞作为兽医科学和动物营养研究平台的机会。
