Feng Guangxue, Liu Jie, Liu Rongrong, Mao Duo, Tomczak Nikodem, Liu Bin
Department of Chemical and Biomolecular Engineering National University of Singapore 4 Engineering Drive 4117585 Singapore.
Institute of Materials Research and Engineering (IMRE) 2 Fusionopolis WayInnovis 136834 Singapore.
Adv Sci (Weinh). 2017 May 1;4(9):1600407. doi: 10.1002/advs.201600407. eCollection 2017 Sep.
Fluorescent and biocompatible organic nanoparticles have attracted great interest in cancer detection and imaging, but the nonspecific cellular uptake has limited the detection specificity and sensitivity. Herein, the authors report the ultrasmall conjugated polymer nanoparticles (CPNs) with bright far-red/near-infrared emission for targeted cancer imaging with high specificity. The sizes of the ultrasmall CPNs are around 6 nm (CPN6), while large CPNs show sizes around 30 nm (CPN30). Moreover, CPN6 exhibits largely improved fluorescence quantum yield (η) of 41% than CPN30 (25%). Benefiting from the ultrasmall size, bare CPN6 shows largely suppressed nonspecific cellular uptake as compared to CPN30, while cyclic arginine-glycine-aspartic acid (cRGD) functionalized CPN6 (cRGD-CPN6) possesses excellent selectivity toward integrin overexpressed MDA-MB-231 cells over other cells in cell mixtures. The faster body clearance of CPN6 over CPN30 indicates its greater potentials as a noninvasive nanoprobe for in vivo and practical applications.
荧光且具有生物相容性的有机纳米颗粒在癌症检测和成像方面引起了极大的关注，但非特异性细胞摄取限制了检测的特异性和灵敏度。在此，作者报道了具有明亮远红/近红外发射的超小共轭聚合物纳米颗粒（CPNs），用于高特异性的靶向癌症成像。超小CPNs的尺寸约为6纳米（CPN6），而大的CPNs尺寸约为30纳米（CPN30）。此外，CPN6的荧光量子产率（η）为41%，相比CPN30（25%）有了很大提高。得益于超小尺寸，与CPN30相比，裸CPN6的非特异性细胞摄取受到很大抑制，而环化精氨酸-甘氨酸-天冬氨酸（cRGD）功能化的CPN6（cRGD-CPN6）在细胞混合物中对整合素过表达的MDA-MB-231细胞具有优于其他细胞的优异选择性。CPN6比CPN30在体内清除更快，这表明其作为用于体内和实际应用的非侵入性纳米探针具有更大的潜力。
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