利用三光子荧光成像实现封装依赖性增强的近红外纳米粒子发射
Encapsulation-Dependent Enhanced Emission of Near-Infrared Nanoparticles Using Three-Photon Fluorescence Imaging.
作者信息
Du Ye, Alifu Nuernisha, Wu Zhiyuan, Chen Runze, Wang Xiaozhen, Ji Guang, Li Qian, Qian Jun, Xu Bin, Song Dong
机构信息
Department of Breast Surgery, The First Hospital, Jilin University, Changchun, China.
State Key Laboratory of Modern Optical Instrumentations, Centre for Optical and Electromagnetic Research, JORCEP (Sino-Swedish Joint Research Center of Photonics), Zhejiang University, Hangzhou, China.
出版信息
Front Bioeng Biotechnol. 2020 Sep 10;8:1029. doi: 10.3389/fbioe.2020.01029. eCollection 2020.
We discovered a unique fluorescent enhancement of dye encapsulated polymeric nanoparticles, which strongly depended on the polymeric matrix. Interestingly, the polymer nanoparticles containing a NIR emissive dye exhibited remarkable enhancement of emission encapsulated by the polymer amphiphilic polymer containing polystyrene (PS) moiety, whereas the nanoparticles showed weak fluorescence when using other polymer encapsulation. The highest fluorescent quantum yield of nanoparticles can reach 27% by using PS-PEG encapsulation, where the strong NIR fluorescence can be observed. These ultra-bright fluorescence nanoparticles also possess a strong three-photon fluorescence and show a good candidate for vascular three-photon fluorescence imaging of mouse brain and ear under 1550 nm fs laser excitation. A fine three-dimensional (3D) reconstruction with an imaging depth of 635 and 180 μm was achieved, respectively. We further demonstrate that these nanoparticles can effectively target the sentinel lymph node (SLN) of mice.
我们发现了一种独特的现象，即染料包封的聚合物纳米颗粒的荧光增强，这在很大程度上取决于聚合物基质。有趣的是，含有近红外发射染料的聚合物纳米颗粒在被含有聚苯乙烯（PS）部分的两亲聚合物包封时表现出显著的发射增强，而使用其他聚合物包封时纳米颗粒显示出较弱的荧光。通过使用PS-PEG包封，纳米颗粒的最高荧光量子产率可达27%，在这种情况下可以观察到强烈的近红外荧光。这些超亮荧光纳米颗粒还具有很强的三光子荧光，并且在1550 nm飞秒激光激发下显示出是小鼠脑和耳的血管三光子荧光成像的良好候选物。分别实现了成像深度为635和180μm的精细三维（3D）重建。我们进一步证明这些纳米颗粒可以有效地靶向小鼠的前哨淋巴结（SLN）。
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