Le Goff C, Cavalier E, Souberbielle J-C, González-Antuña A, Delvin E
Service de Chimie Clinique, CHU de Liège, Belgium.
Service des Explorations Fonctionnelles Hôpital Necker-Enfants Malades, Assistance Publique-Hôpitaux de Paris (AP-HP), Paris, France.
Pract Lab Med. 2015 May 12;2:1-14. doi: 10.1016/j.plabm.2015.04.001. eCollection 2015 Aug 1.
The constantly increasing requests for the measurement of serum 25-hydroxyvitamin D over the last years has led reagent manufacturers to market different automated and semi-automated methods, that being unfortunately not fully harmonized, yield different results. Liquid chromatography coupled to tandem mass spectrometry (LC/MS) has more recently been introduced. This approach allows the distinction between the two forms of 25-hydroxyvitamin D and to measure other metabolites. This approach also requires harmonization to curtail the differences between the different analytical methods. To meet this requirement, the American National Institutes of Health (NIH), the Centre for Disease Control and Prevention (CDC) in Atlanta, the National Institute of Standards and Technology (NIST) and the vitamin D Reference laboratory of Ghent University have pooled their expertise to develop a standardization program. This article reviews the main elements and the difficulties of the automated and semi-automated methods for 25-hydroxyvitamin D, from sample preparation to the analytical phase, as well as those related to mass spectrometry. It also emphasizes the need for standardization to better define the clinical decision thresholds of vitamin D nutritional status.
在过去几年中，对血清25-羟基维生素D检测的需求不断增加，这促使试剂制造商推出了不同的自动化和半自动化检测方法。遗憾的是，这些方法并未完全统一，导致检测结果各异。液相色谱串联质谱法（LC/MS）是最近才引入的。这种方法能够区分25-羟基维生素D的两种形式，并可检测其他代谢产物。该方法同样需要进行标准化，以减少不同分析方法之间的差异。为满足这一要求，美国国立卫生研究院（NIH）、亚特兰大疾病控制与预防中心（CDC）、国家标准与技术研究院（NIST）以及根特大学维生素D参考实验室汇聚各自的专业知识，共同制定了一项标准化计划。本文回顾了从样本制备到分析阶段，25-羟基维生素D自动化和半自动化检测方法的主要内容及难点，以及与质谱相关的内容。文章还强调了标准化对于更好地界定维生素D营养状况临床决策阈值的必要性。
