Musculoskeletal Research Group, IMIM (Hospital del Mar Medical Research Institute), Centro de Investigación Biomédica en Red de Fragilidad y Envejecimiento Saludable (CIBERFES), ISCIII, C/Dr. Aiguader 88, 08003, Barcelona, Spain.
Department of Genetics, Microbiology and Statistics, Facultat de Biologia, Universitat de Barcelona, IBUB, IRSJD, Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), ISCIII, Barcelona, Spain.
Hum Cell. 2018 Jan;31(1):33-41. doi: 10.1007/s13577-017-0181-y. Epub 2017 Sep 20.
Bone tissue is composed of several cell types, which express their own microRNAs (miRNAs) that will play a role in cell function. The set of total miRNAs expressed in all cell types configures the specific signature of the bone tissue in one physiological condition. The aim of this study was to explore the miRNA expression profile of bone tissue from postmenopausal women. Tissue was obtained from trabecular bone and was analyzed in fresh conditions (n = 6). Primary osteoblasts were also obtained from trabecular bone (n = 4) and human osteoclasts were obtained from monocyte precursors after in vitro differentiation (n = 5). MicroRNA expression profiling was obtained for each sample by microarray and a global miRNA analysis was performed combining the data acquired in all the microarray experiments. From the 641 miRNAs detected in bone tissue samples, 346 (54%) were present in osteoblasts and/or osteoclasts. The other 46% were not identified in any of the bone cells analyzed. Intersection of osteoblast and osteoclast arrays identified 101 miRNAs shared by both cell types, which accounts for 30-40% of miRNAs detected in these cells. In osteoblasts, 266 miRNAs were detected, of which 243 (91%) were also present in the total bone array, representing 38% of all bone miRNAs. In osteoclasts, 340 miRNAs were detected, of which 196 (58%) were also present in the bone tissue array, representing 31% of all miRNAs detected in total bone. These analyses provide an overview of miRNAs expressed in bone tissue, broadening our knowledge in the microRNA field.
骨组织由几种细胞类型组成，这些细胞表达自己的 microRNAs（miRNAs），这些 miRNAs 将在细胞功能中发挥作用。在所有细胞类型中表达的总 miRNAs 集合构成了一种生理状态下骨组织的特定特征。本研究旨在探索绝经后妇女骨组织的 miRNA 表达谱。组织取自小梁骨，并在新鲜条件下进行分析（n=6）。还从小梁骨中获得原代成骨细胞（n=4），并通过体外分化从单核细胞前体中获得人破骨细胞（n=5）。通过微阵列对每个样本进行 miRNA 表达谱分析，并对所有微阵列实验获得的数据进行综合分析，进行全局 miRNA 分析。在骨组织样本中检测到的 641 个 miRNAs 中，346 个（54%）存在于成骨细胞和/或破骨细胞中。其余 46%未在任何分析的骨细胞中鉴定到。成骨细胞和破骨细胞阵列的交集确定了 101 个两种细胞类型共有的 miRNAs，占这些细胞中检测到的 miRNAs 的 30-40%。在成骨细胞中，检测到 266 个 miRNAs，其中 243 个（91%）也存在于总骨阵列中，占所有骨 miRNAs 的 38%。在破骨细胞中，检测到 340 个 miRNAs，其中 196 个（58%）也存在于骨组织阵列中，占总骨中检测到的所有 miRNAs 的 31%。这些分析提供了骨组织中表达的 miRNAs 的概述，拓宽了我们在 miRNA 领域的知识。
