Aru Violetta, Balling Engelsen Søren, Savorani Francesco, Culurgioni Jacopo, Sarais Giorgia, Atzori Giulia, Cabiddu Serenella, Cesare Marincola Flaminia
Chemometrics and Analytical Technology, Department of Food Science, University of Copenhagen, 1165 København, Denmark.
Department of Chemical and Geological Sciences, Cittadella Universitaria di Monserrato, 09042 Monserrato, Italy.
Metabolites. 2017 Jul 26;7(3):36. doi: 10.3390/metabo7030036.
In this study, the metabolome of an economically and ecologically important marine bivalve species widely distributed in the Mediterranean region, was characterized by using proton Nuclear Magnetic Resonance (¹H-NMR) spectroscopy. Significant seasonal variations in the content of carbohydrates and free amino acids were observed. The relative amounts of alanine and glycine were found to exhibit the same seasonal pattern as the temperature and salinity at the harvesting site. Several putative sex-specific biomarkers were also discovered. Substantial differences were found for alanine and glycine, whose relative amounts were higher in males, while acetoacetate, choline and phosphocholine were more abundant in female clams. These findings reveal novel insights into the baseline metabolism of the European clam and represent a step forward towards a comprehensive metabolic characterization of the species. Besides providing a holistic view on the prominent nutritional components, the characterization of the metabolome of this bivalve represents an important prerequisite for elucidating the underlying metabolic pathways behind the environment-organism interactions.
在本研究中，利用质子核磁共振（¹H-NMR）光谱对一种在地中海区域广泛分布的具有经济和生态重要性的海洋双壳贝类物种的代谢组进行了表征。观察到碳水化合物和游离氨基酸含量存在显著的季节性变化。发现丙氨酸和甘氨酸的相对含量与收获地点的温度和盐度呈现相同的季节性模式。还发现了几种假定的性别特异性生物标志物。丙氨酸和甘氨酸存在显著差异，其相对含量在雄性中较高，而乙酰乙酸、胆碱和磷酸胆碱在雌性蛤中更为丰富。这些发现揭示了对欧洲蛤基线代谢的新见解，代表了朝着该物种全面代谢特征迈出的一步。除了提供对主要营养成分的整体看法外，这种双壳贝类代谢组的表征是阐明环境与生物相互作用背后潜在代谢途径的重要前提。
