Laboratoire de RMN-RPE, Université Européenne de Bretagne, Université de Bretagne Occidentale UFR Sciences et Techniques, 6 avenue le gorgeu, 29238 BREST Cédex3, France.
Talanta. 2012 Aug 15;97:425-31. doi: 10.1016/j.talanta.2012.04.057. Epub 2012 May 17.
The essence of this study was to investigate the metabolic responses of heart tissues of carpet-shell clam Ruditapes decussatus after exposure to two doses (0.2 and 1 g/L) of Roundup(®) during 24 and 72 h. The main metabolic changes after Roundup(®) exposure were related to disturbance in energy metabolism and metabolic biomarkers such as alanine, succinate, acetate and propionate, suggesting the occcurence of anaerobiosis and the impairment of oxydative metabolism. Results showed also that peak intensities of amino acids used as biomarker of anaerobiosis in molluscs are time and dose dependent. In the opposite, phosphoarginine and ATP level are dependent to Roundup(®) concentration rather than to the time of exposure. We suggest that changes in energy demands require adjustements in the forward arginine kinase reaction rate. Therefore, the results demonstrate the high applicability of HRMAS NMR to elucidate the mechanism of toxicity of Roundup(®). In addition, (31)P HRMAS NMR appeared to be an effective and simple method to follow bioaccumulation of Roundup(®) formulation.
本研究的本质是调查地毯贻贝 Ruditapes decussatus 的心脏组织在暴露于两种剂量（0.2 和 1 g/L）的农达（®）后 24 和 72 小时的代谢反应。暴露于农达（®）后的主要代谢变化与能量代谢和代谢生物标志物（如丙氨酸、琥珀酸、乙酸盐和丙酸盐）的紊乱有关，这表明发生了无氧和氧化代谢的损伤。结果还表明，作为贝类无氧生物标志物的氨基酸的峰强度与时间和剂量有关。相反，磷酸精氨酸和 ATP 水平取决于农达（®）的浓度，而不是暴露时间。我们认为，能量需求的变化需要调整正向精氨酸激酶反应速率。因此，这些结果证明了 HRMAS NMR 在阐明农达（®）毒性机制方面的高适用性。此外，（31）P HRMAS NMR 似乎是一种有效且简单的方法来跟踪农达（®）制剂的生物累积。
