Plymouth Marine Laboratory , Prospect Place, West Hoe, Plymouth, PL1 3DH, U.K.
Environ Sci Technol. 2014 Jun 17;48(12):7044-52. doi: 10.1021/es501601w. Epub 2014 May 29.
Human activities are fundamentally altering the chemistry of the world's oceans. Ocean acidification (OA) is occurring against a background of warming and an increasing occurrence of disease outbreaks, posing a significant threat to marine organisms, communities, and ecosystems. In the current study, (1)H NMR spectroscopy was used to investigate the response of the blue mussel, Mytilus edulis, to a 90-day exposure to reduced seawater pH and increased temperature, followed by a subsequent pathogenic challenge. Analysis of the metabolome revealed significant differences between male and female organisms. Furthermore, males and females are shown to respond differently to environmental stress. While males were significantly affected by reduced seawater pH, increased temperature, and a bacterial challenge, it was only a reduction in seawater pH that impacted females. Despite impacting males and females differently, stressors seem to act via a generalized stress response impacting both energy metabolism and osmotic balance in both sexes. This study therefore has important implications for the interpretation of metabolomic data in mussels, as well as the impact of environmental stress in marine invertebrates in general.
人类活动正在从根本上改变世界海洋的化学物质。在全球变暖以及疾病爆发日益频繁的背景下，海洋酸化正在发生，这对海洋生物、生物群落和生态系统构成了重大威胁。在本研究中，（1）H NMR 光谱用于研究贻贝（Mytilus edulis）在 90 天暴露于降低的海水 pH 值和升高的温度以及随后的病原性挑战下的反应。代谢组学分析显示雌雄个体之间存在显著差异。此外，雌雄个体对环境胁迫的反应也不同。虽然雄性受到海水 pH 值降低、温度升高和细菌挑战的显著影响，但只有海水 pH 值降低才会影响雌性。尽管这些应激源对雌雄贻贝的影响不同，但它们似乎通过影响两性的能量代谢和渗透平衡的普遍应激反应起作用。因此，本研究对贻贝代谢组学数据的解释以及海洋无脊椎动物的环境应激影响具有重要意义。
