United Graduate School of Veterinary Sciences, Yamaguchi University, Yamaguchi 753-8515, Japan.
Shepherd Central Livestock Clinic, Kagoshima 899-1611, Japan.
Toxins (Basel). 2017 Sep 21;9(10):294. doi: 10.3390/toxins9100294.
The objective of this study was to evaluate the metabolic profile of cattle fed with or without zearalenone (ZEN) and sterigmatocystin (STC)-contaminated diets using a gas chromatography-mass spectrometry metabolomics approach. Urinary samples were collected from individual animals ( = 6 per herd) from fattening female Japanese Black (JB) cattle herds (23 months old, 550-600 kg). Herd 1 had persistently high urinary ZEN and STC concentrations due to the presence of contaminated rice straw. Herd 2, the second female JB fattening herd (23 months old, 550-600 kg), received the same dietary feed as Herd 1, with non-contaminated rice straw. Urine samples were collected from Herd 1, two weeks after the contaminated rice straw was replaced with uncontaminated rice straw (Herd 1N). Identified metabolites were subjected to principal component analysis (PCA) and ANOVA. The PCA revealed that the effects on cattle metabolites depended on ZEN and STC concentrations. The contamination of cattle feed with multiple mycotoxins may alter systemic metabolic processes, including metabolites associated with ATP generation, amino acids, glycine-conjugates, organic acids, and purine bases. The results obtained from Herd 1N indicate that a two-week remedy period was not sufficient to improve the levels of urinary metabolites, suggesting that chronic contamination with mycotoxins may have long-term harmful effects on the systemic metabolism of cattle.
本研究旨在采用气相色谱-质谱代谢组学方法评估食用含有玉米赤霉烯酮（ZEN）和杂色曲霉素（STC）污染日粮的牛的代谢特征。从育肥雌性日本黑牛（JB）牛群（23 月龄，550-600kg）的个体动物（每个牛群 6 头）收集尿液样本。牛群 1 的尿液中始终含有高浓度的 ZEN 和 STC，这是由于受污染的稻草的存在。牛群 2 是第二个育肥雌性 JB 牛群（23 月龄，550-600kg），与牛群 1 一样接受相同的饲料喂养，不过使用的是未受污染的稻草。在污染的稻草被更换为未污染的稻草后两周（牛群 1N），从牛群 1 中采集了尿液样本。对鉴定出的代谢物进行主成分分析（PCA）和方差分析（ANOVA）。PCA 结果表明，ZEN 和 STC 浓度对牛代谢物的影响取决于牛的代谢物。牛饲料被多种霉菌毒素污染可能会改变全身代谢过程，包括与 ATP 生成、氨基酸、甘氨酸缀合物、有机酸和嘌呤碱基相关的代谢物。牛群 1N 的结果表明，两周的补救期不足以改善尿液代谢物水平，这表明长期接触霉菌毒素可能对牛的全身代谢产生长期的有害影响。
