基于单量子点的纳米传感器用于快速灵敏检测末端脱氧核苷酸转移酶。
Single quantum dot-based nanosensor for rapid and sensitive detection of terminal deoxynucleotidyl transferase.
作者信息
Wang Li-Juan, Luo Ming-Li, Zhang Qianyi, Tang Bo, Zhang Chun-Yang
机构信息
College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China.
出版信息
Chem Commun (Camb). 2017 Oct 5;53(80):11016-11019. doi: 10.1039/c7cc05485h.
We developed a simple and rapid method for terminal deoxynucleotidyl transferase (TdT) assay on the basis of the polymerization-directed exonuclease-assisted construction of a single quantum dot (QD)-based fluorescence resonance energy transfer (FRET) nanosensor. This method is very sensitive with a detection limit as low as 1 × 10 U μL, and it can be used for the screening of TDT inhibitors and accurate quantification of TdT activity even in 5 cancer cells.
我们基于聚合导向的核酸外切酶辅助构建单量子点（QD）荧光共振能量转移（FRET）纳米传感器，开发了一种用于末端脱氧核苷酸转移酶（TdT）检测的简单快速方法。该方法灵敏度极高，检测限低至1×10 U/μL，甚至可用于筛选TdT抑制剂以及准确量化5个癌细胞中的TdT活性。
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