Ding Xianfeng, Zhang Yuhan, Yang Hongjian, Mao Weimin, Chen Bo, Yang Shifeng, Ding Xiaowen, Zou Dehong, Mo Wenju, He Xiangming, Zhang Xiping
College of Life Science, Zhejiang Sci-Tech University, Hangzhou 310008, Zhejiang Province, China.
Department of Breast Surgery, Zhejiang Cancer Hospital, Hangzhou 310022, Zhejiang Province, China.
Oncotarget. 2017 Apr 21;8(35):58210-58221. doi: 10.18632/oncotarget.17356. eCollection 2017 Aug 29.
Long non-coding RNAs (lncRNAs) have been shown to play important regulatory role in certain type of cancers biology, including breast and lung cancers. However, the lncRNA expression in breast cancer combined with primary lung cancer remains unknown. In this study, databases of the Cancer Genome Atlas (TCGA) and the lncRNA profiler of contained candidate 192 lncRNAs were utilized. 11 lncRNAs were differentially expressed in breast cancer, 9 candidate lncRNAs were differentially expressed in lung cancer. In order to find the aberrant expression of lncRNAs in breast cancer combined with primary lung cancer, seven samples of primary breast cancer and lung cancer were studied for the expression of selected lncRNAs. The results showed that SNHG6 and NEAT1 were reversely expressed in breast cancer combined with primary lung cancer compared with primary breast or lung cancer. In addition, a significant correlation of lncRNAs was found in the patients whose age was above 56 in breast cancer. What's more, PVT1 expression was negatively correlated with the pathological stage, and the level of ER, PR, HER2, p53 in breast cancer. Furthermore, lncRNA expression did not have significant relationship with the 5-year survival of patients with breast cancer combined with primary lung cancer. The findings revealed that PVT1, SNHG6, NEAT1 may serve as a prognostic marker for breast cancer combined with primary lung cancer. Therefore, these lncRNAs are potential molecular indicators in the diagnosis and prognosis of cancer in the future.
长链非编码RNA（lncRNAs）已被证明在某些类型的癌症生物学中发挥重要的调控作用，包括乳腺癌和肺癌。然而，乳腺癌合并原发性肺癌中的lncRNA表达情况仍不清楚。在本研究中，利用了癌症基因组图谱（TCGA）数据库以及包含192个候选lncRNAs的lncRNA分析器。11个lncRNAs在乳腺癌中差异表达，9个候选lncRNAs在肺癌中差异表达。为了找出乳腺癌合并原发性肺癌中lncRNAs的异常表达情况，对7例原发性乳腺癌和肺癌样本进行了所选lncRNAs表达的研究。结果显示，与原发性乳腺癌或肺癌相比，SNHG6和NEAT1在乳腺癌合并原发性肺癌中呈反向表达。此外，在年龄大于56岁的乳腺癌患者中发现lncRNAs存在显著相关性。而且，PVT1表达与乳腺癌的病理分期以及ER、PR、HER2、p53水平呈负相关。此外，lncRNA表达与乳腺癌合并原发性肺癌患者的5年生存率没有显著关系。这些发现表明，PVT1、SNHG6、NEAT1可能作为乳腺癌合并原发性肺癌的预后标志物。因此，这些lncRNAs是未来癌症诊断和预后的潜在分子指标。
