希腊西北部奶制品生产链中金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌（MRSA）的流行情况。
Prevalence of Staphylococcus aureus and of methicillin-resistant S. aureus (MRSA) along the production chain of dairy products in north-western Greece.
机构信息
Laboratory of Hygiene of Foods of Animal Origin, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Greek Agricultural Organization-DIMITRA, Veterinary Research Institute of Thessaloniki, 57006 Thermi, Greece.
出版信息
Food Microbiol. 2018 Feb;69:43-50. doi: 10.1016/j.fm.2017.07.016. Epub 2017 Jul 28.
The objective of this study was to estimate the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in the production chain of dairy products. Of 367 tested samples (36 bulk tank milk (BTM), 19 dairy products, 72 human, 185 animal, 55 equipment), 212 (57.8%) were found positive for S. aureus. Almost all isolates (99.6%) were resistant to at least one antimicrobial and 13.3% were multi-drug resistant (MDR), exhibiting resistance to three or more antibiotic classes. Eleven samples (3%) were found contaminated by MRSA carrying the mecA gene. None of the MRSA isolates carried the mecC or the Pandon-Valentine leucocidin (PVL) genes. Four spa types were identified among the MRSA isolates: t127, t3586, t1773, t4038, with t127 being the most prevalent (7 out of 11). Two of them, t3586 and t1773, were isolated for the first time in Greece. Furthermore, Pulse-Field Gel Electrophoresis (PFGE) analysis indicated clonal circulation through the dairy production chain. The presence of MDR S. aureus, and especially MRSA, in animals and dairy products represents a potential threat for the spread of this pathogen in the community. The results indicated that human, animal and environmental sources could be involved in the contamination of dairy products along their production chain and therefore further investigation of contamination sources is needed to control the dispersion of MRSA in the community.
本研究旨在估计乳制品生产链中耐甲氧西林金黄色葡萄球菌（MRSA）的流行情况。在 367 个测试样本（36 个散装奶（BTM）、19 个乳制品、72 个人体、185 个动物、55 个设备）中，212 个（57.8%）被检测出金黄色葡萄球菌阳性。几乎所有的分离株（99.6%）至少对一种抗菌药物具有耐药性，13.3%为多药耐药（MDR），对三种或更多抗生素类别具有耐药性。有 11 个样本（3%）被发现被携带 mecA 基因的 MRSA 污染。MRSA 分离株均未携带 mecC 或 Pandon-Valentine 白细胞毒素（PVL）基因。在 MRSA 分离株中鉴定出 4 种 spa 型：t127、t3586、t1773、t4038，其中 t127 最为流行（11 个中有 7 个）。其中 2 种，t3586 和 t1773，是在希腊首次分离到的。此外，脉冲场凝胶电泳（PFGE）分析表明，MRSA 通过乳制品生产链进行克隆循环。动物和乳制品中存在 MDR 金黄色葡萄球菌，尤其是 MRSA，代表了这种病原体在社区中传播的潜在威胁。结果表明，人类、动物和环境来源可能参与了乳制品在生产链中的污染，因此需要进一步调查污染来源，以控制 MRSA 在社区中的扩散。
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