Laboratory of Hygiene of Foods of Animal Origin-Veterinary Public Health, School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece.
Laboratory of Safety and Quality of Milk and Dairy Products, School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece.
Food Microbiol. 2019 Dec;84:103249. doi: 10.1016/j.fm.2019.103249. Epub 2019 Jun 22.
Recently, there has been an increased tendency towards raw-milk consumption, which may pose a consumer risk, due to the possible presence of human pathogenic microorganisms, such as Staphylococcus aureus and even methicillin-resistant S. aureus (MRSA). The prevalence of S. aureus and methicillin-resistant S. aureus (MRSA) was investigated in 40 dairy (cattle, sheep and goat) farms in northern Greece. S. aureus and MRSA were detected in 47.8% and 4.1% of the 387 samples (raw milk, farmers and animal samples) tested, respectively. Most (81.3%) of the MRSA isolates harbored the mecA gene, whereas the mecC or Panton-Valentine Leucocidin (PVL) genes were not detected. Seven spa types were identified, with t127 being the most prevalent. Spa type t034 (CC398) was isolated for the first time from livestock in Greece. Staphylococcal enterotoxin genes were detected in 93.8% of the MRSA isolates. The MRSA isolates were genetically diverse and were all capable of biofilm production. Our results confirm the lurking threat of MRSA in raw milk and dairy farms and suggest the need for surveillance programs starting at the farm level.
最近，人们越来越倾向于饮用生奶，由于可能存在金黄色葡萄球菌等人类病原菌，甚至耐甲氧西林金黄色葡萄球菌（MRSA），这可能会对消费者健康造成风险。本研究在希腊北部的 40 家奶牛（牛、绵羊和山羊）养殖场中调查了金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌（MRSA）的流行情况。在检测的 387 份样本（生奶、农民和动物样本）中，金黄色葡萄球菌和 MRSA 的检出率分别为 47.8%和 4.1%。大多数（81.3%）MRSA 分离株携带 mecA 基因，而 mecC 或 Panton-Valentine Leucocidin（PVL）基因未检出。鉴定出 7 种 spa 型，t127 最为流行。spa 型 t034（CC398）首次从希腊的牲畜中分离出来。93.8%的 MRSA 分离株携带肠毒素基因。MRSA 分离株具有遗传多样性，均具有生物膜形成能力。我们的研究结果证实了 MRSA 在生奶和奶牛场中的潜在威胁，并表明需要从农场层面开始进行监测计划。
