中华大蟾蜍晚期胚胎分离出的线粒体对单胺氧化酶活性的影响。
Changes in monoamine oxidase activity by mitochondria isolated from late embryos of Bufo bufo.
作者信息
Nicotra A, Senatori O
机构信息
Dipartimento di Biologia Animale e dell'Uomo, Università di Roma, Italy.
出版信息
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1988;89(1):5-9. doi: 10.1016/0742-8413(88)90138-7.
PMID:2894273
Abstract
- Monoamine oxidase activity has been assayed in mitochondria isolated from post-neural embryos (stages 14-25) of Bufo bufo, using 5-HT and PEA as substrates. 2. Mitochondria isolated from stages 19 to 25 show an increasing ability in deaminating monoamines, PEA being metabolized at a higher level with respect to 5-HT. 3. At all the examined stages 5-HT is metabolized by an enzyme corresponding to MAO A, while PEA, from stage 19, is largely deaminated by a semicarbazide-sensitive amine oxidase (SSAO). 4. The effect of Triton X-100 on MAO A activity appears remarkably different in mitochondria isolated from embryos at stages 14 and 25 respectively.
摘要
- 以5-羟色胺（5-HT）和苯乙胺（PEA）为底物，对从中华大蟾蜍神经胚后期（14-25期）分离出的线粒体中的单胺氧化酶活性进行了测定。2. 从19期到25期分离出的线粒体显示出脱氨单胺的能力增强，相对于5-HT，PEA的代谢水平更高。3. 在所有检测阶段，5-HT均由与单胺氧化酶A（MAO A）相对应的一种酶代谢，而从19期开始，PEA主要由氨基脲敏感胺氧化酶（SSAO）脱氨。4. 曲拉通X-100对MAO A活性的影响在分别从14期和25期胚胎分离出的线粒体中表现出明显不同。
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