蟾蜍卵线粒体中A型和B型单胺氧化酶活性的存在情况。

Occurrence of type A and type B monoamine oxidase activities in toad egg mitochondria.

作者信息

Nicotra A, Senatori O

出版信息

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1984;78(2):353-6. doi: 10.1016/0742-8413(84)90097-5.

Abstract

Monoamine oxidase activity has been assayed radiochemically in mitochondria from Bufo bufo eggs. Time courses of MAO activity towards PEA and 5-HT indicate that when PEA is used as a substrate higher specific activities are obtained. The inhibition patterns by clorgyline and deprenyl demonstrate that both type A and type B MAO are present. Type A activity is more sensitive than type B to the inhibitory effect of Triton X-100. Low concentrations of 2-mercaptoethanol cause a slight stimulation of type A and B activities. Increasing concentrations result in a decrease of activity.

摘要

已经采用放射化学方法测定了中华大蟾蜍卵线粒体中的单胺氧化酶活性。单胺氧化酶对苯乙胺（PEA）和5-羟色胺（5-HT）的活性随时间变化过程表明，当以PEA作为底物时可获得更高的比活性。氯吉兰和司来吉兰的抑制模式表明，A型和B型单胺氧化酶均存在。A型活性比B型对 Triton X-100的抑制作用更敏感。低浓度的2-巯基乙醇会轻微刺激A型和B型活性。浓度增加则导致活性降低。