School of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China.
State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
Bioresour Technol. 2018 Aug;262:177-183. doi: 10.1016/j.biortech.2018.04.077. Epub 2018 Apr 22.
Upgrading of biomass-derived 5-hydroxymethylfurfural (HMF) has attracted considerable interest recently. In this work, efficient synthesis of 2,5-bis(hydroxymethyl)furan (BHMF) from HMF was reported with the acclimatized Meyerozyma guilliermondii SC1103 cells entrapped in calcium alginate beads. Catalytic activities of the cells as well as their HMF-tolerant level increased significantly upon acclimatization and immobilization. BHMF was obtained within 7-24 h with good yields (82-85%) and excellent selectivities (99%) when the substrate concentrations were 200-300 mM. In scale-up synthesis, BHMF of up to 181 mM was produced within 7 h, and its productivity was approximately 3.3 g/L h. In addition, the immobilized biocatalyst showed satisfactory operational stability; the cell viability of 70% was retained after reuse 4 times. With rice straw hydrolysate as co-substrate, both the reaction rate and selectivity decreased, likely due to the deleterious influence of xylose in the hydrolysate.
最近，从生物质衍生的 5-羟甲基糠醛（HMF）升级引起了相当大的关注。在这项工作中，报道了用驯化的 Meyerozyma guilliermondii SC1103 细胞包埋在海藻酸钙珠中来高效合成 2,5-双（羟甲基）呋喃（BHMF）。驯化和固定化后，细胞的催化活性及其对 HMF 的耐受性水平显著提高。当底物浓度为 200-300 mM 时，BHMF 在 7-24 h 内即可获得良好的收率（82-85%）和优异的选择性（99%）。在放大合成中，在 7 h 内可生产高达 181 mM 的 BHMF，其生产率约为 3.3 g/L/h。此外，固定化生物催化剂表现出令人满意的操作稳定性；重复使用 4 次后，细胞活力保持在 70%。用稻草水解物作为共底物时，反应速率和选择性都降低了，可能是由于水解物中木糖的有害影响。
