• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

基于内体相关蛋白分选的方法得到均一的人多能干细胞源性多巴胺能前体细胞用于移植。

IAP-Based Cell Sorting Results in Homogeneous Transplantable Dopaminergic Precursor Cells Derived from Human Pluripotent Stem Cells.

机构信息

Miltenyi Biotec GmbH, 51429 Bergisch Gladbach, Germany.

Aix-Marseille University, CNRS, IBDM; Campus de Luminy, 13009 Marseille, France.

出版信息

Stem Cell Reports. 2017 Oct 10;9(4):1207-1220. doi: 10.1016/j.stemcr.2017.08.016. Epub 2017 Sep 21.

DOI:10.1016/j.stemcr.2017.08.016
PMID:28943253
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5639383/
Abstract

Human pluripotent stem cell (hPSC)-derived mesencephalic dopaminergic (mesDA) neurons can relieve motor deficits in animal models of Parkinson's disease (PD). Clinical translation of differentiation protocols requires standardization of production procedures, and surface-marker-based cell sorting is considered instrumental for reproducible generation of defined cell products. Here, we demonstrate that integrin-associated protein (IAP) is a cell surface marker suitable for enrichment of hPSC-derived mesDA progenitor cells. Immunomagnetically sorted IAP mesDA progenitors showed increased expression of ventral midbrain floor plate markers, lacked expression of pluripotency markers, and differentiated into mature dopaminergic (DA) neurons in vitro. Intrastriatal transplantation of IAP cells sorted at day 16 of differentiation in a rat model of PD resulted in functional recovery. Grafts from sorted IAP mesDA progenitors were more homogeneous in size and DA neuron density. Thus, we suggest IAP-based sorting for reproducible prospective enrichment of mesDA progenitor cells in clinical cell replacement strategies.

摘要

人多能干细胞（hPSC）衍生的中脑多巴胺能（mesDA）神经元可缓解帕金森病（PD）动物模型的运动功能障碍。分化方案的临床转化需要生产程序的标准化，而基于表面标志物的细胞分选被认为是可重复产生定义明确的细胞产物的关键。在这里，我们证明整合素相关蛋白（IAP）是一种适合富集 hPSC 衍生的 mesDA 祖细胞的细胞表面标志物。免疫磁珠分选的 IAP mesDA 祖细胞表现出中脑腹侧基板标记物的表达增加，缺乏多能性标记物的表达，并在体外分化为成熟的多巴胺能（DA）神经元。在 PD 大鼠模型中，在分化的第 16 天分选的 IAP 细胞进行纹状体内移植导致功能恢复。从分选的 IAP mesDA 祖细胞中获得的移植物在大小和 DA 神经元密度上更加均匀。因此，我们建议使用基于 IAP 的分选方法，在临床细胞替代策略中可重复地富集 mesDA 祖细胞。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dac/5639383/43362258ade4/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dac/5639383/ca5cf5ee4d8e/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dac/5639383/ebe3d2e46f1c/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dac/5639383/c39c74095143/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dac/5639383/60e2ed337388/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dac/5639383/53f5f5d11d54/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dac/5639383/eb3a3dce1e4b/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dac/5639383/43362258ade4/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dac/5639383/ca5cf5ee4d8e/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dac/5639383/ebe3d2e46f1c/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dac/5639383/c39c74095143/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dac/5639383/60e2ed337388/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dac/5639383/53f5f5d11d54/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dac/5639383/eb3a3dce1e4b/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7dac/5639383/43362258ade4/gr6.jpg

相似文献

1
IAP-Based Cell Sorting Results in Homogeneous Transplantable Dopaminergic Precursor Cells Derived from Human Pluripotent Stem Cells.基于内体相关蛋白分选的方法得到均一的人多能干细胞源性多巴胺能前体细胞用于移植。
Stem Cell Reports. 2017 Oct 10;9(4):1207-1220. doi: 10.1016/j.stemcr.2017.08.016. Epub 2017 Sep 21.
2
FolR1: a novel cell surface marker for isolating midbrain dopamine neural progenitors and nascent dopamine neurons.FolR1：一种新型的细胞表面标志物，用于分离中脑多巴胺神经前体细胞和新生多巴胺神经元。
Sci Rep. 2016 Sep 1;6:32488. doi: 10.1038/srep32488.
3
Identification of transplantable dopamine neuron precursors at different stages of midbrain neurogenesis.中脑神经发生不同阶段可移植多巴胺能神经元前体的鉴定。
Exp Neurol. 2009 Sep;219(1):341-54. doi: 10.1016/j.expneurol.2009.06.006. Epub 2009 Jun 23.
4
Differences in neurogenic potential in floor plate cells along an anteroposterior location: midbrain dopaminergic neurons originate from mesencephalic floor plate cells.沿前后位置的底板细胞神经源性潜能差异：中脑多巴胺能神经元起源于中脑底板细胞。
Development. 2007 Sep;134(17):3213-25. doi: 10.1242/dev.02879. Epub 2007 Aug 1.
5
Identifying the optimal developmental age of human pluripotent stem cell-derived midbrain dopaminergic progenitors for transplantation in a rodent model of Parkinson's disease.鉴定人类多能干细胞衍生的中脑多巴胺能祖细胞在帕金森病啮齿动物模型中移植的最佳发育年龄。
Exp Neurol. 2022 Dec;358:114219. doi: 10.1016/j.expneurol.2022.114219. Epub 2022 Aug 30.
6
Transcriptome analysis reveals transmembrane targets on transplantable midbrain dopamine progenitors.转录组分析揭示了可移植中脑多巴胺祖细胞上的跨膜靶点。
Proc Natl Acad Sci U S A. 2015 Apr 14;112(15):E1946-55. doi: 10.1073/pnas.1501989112. Epub 2015 Mar 9.
7
Isolation of human induced pluripotent stem cell-derived dopaminergic progenitors by cell sorting for successful transplantation.通过细胞分选分离人诱导多能干细胞衍生的多巴胺能祖细胞，以实现成功移植。
Stem Cell Reports. 2014 Mar 6;2(3):337-50. doi: 10.1016/j.stemcr.2014.01.013. eCollection 2014 Mar 11.
8
Isolation of LMX1a Ventral Midbrain Progenitors Improves the Safety and Predictability of Human Pluripotent Stem Cell-Derived Neural Transplants in Parkinsonian Disease.LMX1a 腹侧中脑神经祖细胞的分离提高了人多能干细胞源性神经移植在帕金森病中的安全性和可预测性。
J Neurosci. 2019 Nov 27;39(48):9521-9531. doi: 10.1523/JNEUROSCI.1160-19.2019. Epub 2019 Oct 22.
9
Engraftable Induced Pluripotent Stem Cell-Derived Neural Precursors for Brain Repair.可植入诱导多能干细胞衍生的神经前体细胞用于脑修复。
Methods Mol Biol. 2020;2155:23-39. doi: 10.1007/978-1-0716-0655-1_3.
10
Improved cell therapy protocols for Parkinson's disease based on differentiation efficiency and safety of hESC-, hiPSC-, and non-human primate iPSC-derived dopaminergic neurons.基于人胚胎干细胞（hESC）、人诱导多能干细胞（hiPSC）和非人类灵长类动物 iPSC 来源的多巴胺能神经元的分化效率和安全性改进的帕金森病细胞治疗方案。
Stem Cells. 2013 Aug;31(8):1548-62. doi: 10.1002/stem.1415.

引用本文的文献

1
TNF-NF-κB-p53 axis restricts in vivo survival of hPSC-derived dopamine neurons.TNF-NF-κB-p53 轴限制人胚胎干细胞源性多巴胺神经元在体内的存活。
Cell. 2024 Jul 11;187(14):3671-3689.e23. doi: 10.1016/j.cell.2024.05.030. Epub 2024 Jun 11.
2
Cell reprogramming therapy for Parkinson's disease.帕金森病的细胞重编程疗法
Neural Regen Res. 2024 Nov 1;19(11):2444-2455. doi: 10.4103/1673-5374.390965. Epub 2023 Dec 15.
3
Current Status and Future Perspectives on Stem Cell-Based Therapies for Parkinson's Disease.基于干细胞的帕金森病治疗的现状与未来展望

本文引用的文献

1
Predictive Markers Guide Differentiation to Improve Graft Outcome in Clinical Translation of hESC-Based Therapy for Parkinson's Disease.预测性标志物指导分化以改善基于人胚胎干细胞的帕金森病治疗临床转化中的移植物结局。
Cell Stem Cell. 2017 Jan 5;20(1):135-148. doi: 10.1016/j.stem.2016.09.004. Epub 2016 Oct 27.
2
Purification of functional human ES and iPSC-derived midbrain dopaminergic progenitors using LRTM1.使用 LRTM1 对功能性人胚胎干细胞和 iPSC 来源的中脑多巴胺能祖细胞进行纯化。
Nat Commun. 2016 Oct 14;7:13097. doi: 10.1038/ncomms13097.
3
Molecular Diversity of Midbrain Development in Mouse, Human, and Stem Cells.
J Mov Disord. 2023 Jan;16(1):22-41. doi: 10.14802/jmd.22141. Epub 2023 Jan 12.
4
Neuronal cell-based medicines from pluripotent stem cells: Development, production, and preclinical assessment.基于多能干细胞的神经细胞药物：开发、生产和临床前评估。
Stem Cells Transl Med. 2021 Nov;10 Suppl 2(Suppl 2):S31-S40. doi: 10.1002/sctm.20-0522.
5
Trophoblast glycoprotein is a marker for efficient sorting of ventral mesencephalic dopaminergic precursors derived from human pluripotent stem cells.滋养层糖蛋白是用于高效分选源自人类多能干细胞的腹侧中脑多巴胺能前体细胞的标志物。
NPJ Parkinsons Dis. 2021 Jul 19;7(1):61. doi: 10.1038/s41531-021-00204-8.
6
Chemical and Biological Molecules Involved in Differentiation, Maturation, and Survival of Dopaminergic Neurons in Health and Parkinson's Disease: Physiological Aspects and Clinical Implications.参与健康及帕金森病中多巴胺能神经元分化、成熟和存活的化学及生物分子：生理方面及临床意义
Biomedicines. 2021 Jun 29;9(7):754. doi: 10.3390/biomedicines9070754.
7
Neural tube patterning: from a minimal model for rostrocaudal patterning toward an integrated 3D model.神经管模式形成：从用于头尾模式形成的最小模型到综合三维模型
iScience. 2021 May 20;24(6):102559. doi: 10.1016/j.isci.2021.102559. eCollection 2021 Jun 25.
8
Human stem cells harboring a suicide gene improve the safety and standardisation of neural transplants in Parkinsonian rats.携带自杀基因的人类干细胞提高了帕金森病大鼠神经移植的安全性和标准化。
Nat Commun. 2021 May 27;12(1):3275. doi: 10.1038/s41467-021-23125-9.
9
Gene therapy restores dopamine transporter expression and ameliorates pathology in iPSC and mouse models of infantile parkinsonism.基因治疗可恢复多巴胺转运体的表达，并改善 iPSC 和婴儿帕金森病小鼠模型的病理。
Sci Transl Med. 2021 May 19;13(594). doi: 10.1126/scitranslmed.aaw1564.
10
The potential therapy with dental tissue-derived mesenchymal stem cells in Parkinson's disease.牙科组织来源的间充质干细胞在帕金森病中的潜在治疗作用
Stem Cell Res Ther. 2021 Jan 6;12(1):5. doi: 10.1186/s13287-020-01957-4.
小鼠、人类及干细胞中脑发育的分子多样性
Cell. 2016 Oct 6;167(2):566-580.e19. doi: 10.1016/j.cell.2016.09.027.
4
Cell-based therapies for Parkinson disease—past insights and future potential.基于细胞的帕金森病疗法——过去的见解和未来的潜力。
Nat Rev Neurol. 2015 Sep;11(9):492-503. doi: 10.1038/nrneurol.2015.123. Epub 2015 Aug 4.
5
Transcriptome analysis reveals transmembrane targets on transplantable midbrain dopamine progenitors.转录组分析揭示了可移植中脑多巴胺祖细胞上的跨膜靶点。
Proc Natl Acad Sci U S A. 2015 Apr 14;112(15):E1946-55. doi: 10.1073/pnas.1501989112. Epub 2015 Mar 9.
6
Optogenetics enables functional analysis of human embryonic stem cell-derived grafts in a Parkinson's disease model.光遗传学能够在帕金森病模型中对人胚胎干细胞衍生移植物进行功能分析。
Nat Biotechnol. 2015 Feb;33(2):204-9. doi: 10.1038/nbt.3124. Epub 2015 Jan 12.
7
Human ESC-derived dopamine neurons show similar preclinical efficacy and potency to fetal neurons when grafted in a rat model of Parkinson's disease.当移植到帕金森病大鼠模型中时，人胚胎干细胞衍生的多巴胺神经元显示出与胎儿神经元相似的临床前疗效和效力。
Cell Stem Cell. 2014 Nov 6;15(5):653-65. doi: 10.1016/j.stem.2014.09.017.
8
jvenn: an interactive Venn diagram viewer.jvenn：一个交互式的韦恩图查看器。
BMC Bioinformatics. 2014 Aug 29;15(1):293. doi: 10.1186/1471-2105-15-293.
9
Selection Based on FOXA2 Expression Is Not Sufficient to Enrich for Dopamine Neurons From Human Pluripotent Stem Cells.基于FOXA2表达的筛选不足以富集来自人类多能干细胞的多巴胺能神经元。
Stem Cells Transl Med. 2014 Sep;3(9):1032-42. doi: 10.5966/sctm.2014-0011. Epub 2014 Jul 14.
10
Optical control of muscle function by transplantation of stem cell-derived motor neurons in mice.通过在小鼠中移植干细胞衍生的运动神经元来实现肌肉功能的光学控制。
Science. 2014 Apr 4;344(6179):94-7. doi: 10.1126/science.1248523.