Graduate Program in Dentistry, School of Life Sciences, Pontifícia Universidade Católica do Paraná, Curitiba, Brazil.
Oral Dis. 2018 Apr;24(3):372-376. doi: 10.1111/odi.12784. Epub 2017 Oct 23.
To identify the impact of MUC5B polymorphisms on dental caries.
A case-control study was performed with patients recruited at Pontifícia Universidade Católica do Paraná. These individuals were aged 12 years old or more. Dental caries was diagnosed using the International Caries Detection and Assessment System, and the effects of socioeconomic, dietary, and hygiene factors on dental caries were investigated. Furthermore, buccal cells were collected, and their DNA was extracted and amplified using PCR. Uni-, bi-, and multivariate analyses were performed.
Two hundred patients were recruited, 100 were assigned to the case group and 100 to the control group. In the bivariate analysis, the following variables showed significant results: ethnicity (p = .008), biofilm (p < .001), and gingivitis (p < .001). The MUC5B gene affected dental caries with the markers rs2735733 (p < .001), rs2249073 (p < .001), and rs2857476 (p < .001). In the multivariate analysis, the biofilm variable remained significant (p = .026), as did the following markers from the MUC5B gene: rs2735733 (p = .019), rs2249073 (p < .025), and rs2857476 (p < .005).
Genetic variations in the MUC5B gene can influence dental caries.
确定 MUC5B 多态性对龋齿的影响。
采用病例对照研究，在巴西帕拉那天主教大学招募患者。这些患者年龄在 12 岁及以上。使用国际龋齿检测和评估系统诊断龋齿，并研究社会经济、饮食和卫生因素对龋齿的影响。此外，采集颊细胞，使用 PCR 提取和扩增其 DNA。进行单变量、双变量和多变量分析。
共招募了 200 名患者，其中 100 名患者被分配到病例组，100 名患者被分配到对照组。在双变量分析中，以下变量显示出显著结果：种族（p=0.008）、生物膜（p<0.001）和牙龈炎（p<0.001）。MUC5B 基因受 rs2735733（p<0.001）、rs2249073（p<0.001）和 rs2857476（p<0.001）标记物的影响与龋齿有关。在多变量分析中，生物膜变量仍然显著（p=0.026），MUC5B 基因的以下标记物也具有显著意义：rs2735733（p=0.019）、rs2249073（p<0.025）和 rs2857476（p<0.005）。
MUC5B 基因的遗传变异可能影响龋齿。
