Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
Second Pharmacology Laboratory, Institute of Materia Medica, Urumqi, Xinjiang 830000, P.R. China.
Mol Med Rep. 2017 Nov;16(5):7213-7220. doi: 10.3892/mmr.2017.7566. Epub 2017 Sep 20.
Lucilia sericata maggots have beneficial properties; however, their protective effects on burn wounds have yet to be fully elucidated. In the present study, a deep second‑degree burn rat model was used to investigate the burn wound healing properties of aqueous extract of maggots (MAE). The anti‑inflammatory, antioxidative and antibacterial activities were examined. In addition, the protein expression levels of Akt, vascular endothelial growth factor A (VEGFA) and nuclear factor‑κB (NF‑κB) were detected by western blotting. The findings of the present study revealed that MAE treatment increased burn wound healing and hydroxyproline content in the burn‑treated rats. A total of seven compounds (MAE‑P1‑P7) were separated from MAE and a comparative study was performed to identify the major active component. The results demonstrated that MAE‑P6 exerted greater antibacterial activity compared with the other compounds. MAE‑P6 treatment reduced tissue levels of malondialdehyde, tumor necrosis factor‑α and interleukin‑6, and increased superoxide dismutase activity. Furthermore, MAE‑P6 increased the expression levels of VEGFA and reduced NF‑κB expression through Akt, which was verified by treatment with the Akt‑specific inhibitor, LY294002. In conclusion, the present study demonstrated that the beneficial effects of MAE on burn wound healing were due to its antibacterial, antioxidative and anti‑inflammatory activities. MAE‑P6 reduced the release of inflammatory cytokines via the Akt/NF‑κB signaling pathway, and regulated angiogenesis and vasopermeability via the Akt/VEGFA pathway.
丝光绿蝇幼虫具有有益特性；然而，其对烧伤创面的保护作用尚未完全阐明。在本研究中，建立了深二度烧伤大鼠模型，以研究蝇蛆水提物（MAE）对烧伤创面的愈合作用。检测了其抗炎、抗氧化和抗菌活性。此外，通过蛋白质印迹法检测 Akt、血管内皮生长因子 A（VEGFA）和核因子-κB（NF-κB）的蛋白表达水平。本研究结果表明，MAE 治疗可增加烧伤大鼠的烧伤创面愈合和羟脯氨酸含量。从 MAE 中分离出 7 种化合物（MAE-P1-P7），并进行了比较研究以鉴定主要活性成分。结果表明，MAE-P6 与其他化合物相比具有更强的抗菌活性。MAE-P6 治疗可降低组织中丙二醛、肿瘤坏死因子-α和白细胞介素-6 的水平，提高超氧化物歧化酶的活性。此外，MAE-P6 通过 Akt 增加 VEGFA 的表达水平并降低 NF-κB 的表达，这一作用可通过 Akt 特异性抑制剂 LY294002 进行验证。综上所述，本研究表明 MAE 对烧伤创面愈合的有益作用与其抗菌、抗氧化和抗炎活性有关。MAE-P6 通过 Akt/NF-κB 信号通路减少炎症细胞因子的释放，并通过 Akt/VEGFA 通路调节血管生成和血管通透性。
