Department of Obstetrics and Gynecology, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210004, P.R. China.
Department of Human Resources, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210004, P.R. China.
Mol Med Rep. 2017 Nov;16(5):7337-7344. doi: 10.3892/mmr.2017.7582. Epub 2017 Sep 21.
Preeclampsia (PE), a life‑threatening, complicated pregnancy‑associated disease, has recently become a research focus in obstetrics. However, the peptidome of the amniotic fluid in PE patients has rarely been investigated. The present study used peptidomic profiling to perform a comparative analysis of human amniotic fluid between normal and PE pregnancies. Centrifugal ultrafiltration and liquid chromatography‑tandem mass spectrometry (LC‑MS/MS) was combined with isotopomeric dimethyl labels to gain a deeper understanding of the role of proteins and the peptidome in the onset of PE. Following ultrafiltration and LC‑MS/MS, 352 peptides were identified. Of these, 23 peptides were observed to be significantly differentially expressed (6 downregulated and 17 upregulated; P<0.05). Using Gene Ontology and Blastp analyses, the functions and biological activities of these 23 peptides were identified and revealed to include autophagy, signal transduction, receptor activity, enzymatic activity and nucleic acid binding. In addition, a bibliographic search revealed that some of the identified peptides, including Titin, are crucial to the pathogenesis underlying PE. The present study identified 23 peptides expressed at significantly different levels in the amniotic fluid of PE and normal pregnancies. A comprehensive peptidome analysis is more efficient than a simple biomarker analysis at revealing deficiencies and improving the detection rate in diseases. These analyses therefore provide a substantial advantage in applications aimed at the discovery of disease‑specific biomarkers.
子痫前期（PE）是一种危及生命的、复杂的妊娠相关疾病，最近已成为产科研究的重点。然而，PE 患者的羊水肽组很少被研究。本研究使用肽组学分析方法对正常妊娠和 PE 妊娠的人羊水进行了比较分析。离心超滤和液相色谱-串联质谱（LC-MS/MS）联合同位素二甲基标记，以更深入地了解蛋白质和肽组在 PE 发病中的作用。超滤和 LC-MS/MS 后，鉴定出 352 种肽。其中，23 种肽表现出明显的差异表达（下调 6 种，上调 17 种；P<0.05）。通过基因本体论和 Blastp 分析，鉴定出这 23 种肽的功能和生物学活性，包括自噬、信号转导、受体活性、酶活性和核酸结合。此外，文献检索表明，一些鉴定出的肽，包括 Titin，对 PE 的发病机制至关重要。本研究鉴定出在 PE 和正常妊娠的羊水中表达水平显著不同的 23 种肽。与简单的生物标志物分析相比，全面的肽组分析在揭示疾病中的缺陷和提高检测率方面更有效。因此，这些分析在发现疾病特异性生物标志物的应用中具有很大的优势。
