Nóbrega Fernanda S, Selim Mariana B, Arana-Chavez Victor E, Correa Luciana, Ferreira Márcio P, Zoppa André L V
Am J Vet Res. 2017 Oct;78(10):1210-1214. doi: 10.2460/ajvr.78.10.1210.
OBJECTIVE To evaluate the efficacy of castor oil polyurethane polymer with calcium carbonate for use in a unicortical ostectomy on the dorsal surface of the third metacarpal bone of horses. ANIMALS 6 adult horses. PROCEDURES A unicortical ostectomy was created on the dorsal surface of both third metacarpal bones of each horse. Castor bean (Ricinus communis) oil polyurethane polymer with calcium carbonate was implanted into the ostectomy on 1 limb, and the ostectomy of the contralateral limb was left unfilled and served as a control sample. Ostectomy sites were evaluated histologically 120 days later. Biopsy specimens were obtained from the interface of bone and polymer or the interface of bone and newly formed tissue; specimens were processed for histomorphometric evaluation by use of light microscopy, immunohistochemical analysis, histochemical analysis, and transmission electron microscopy. RESULTS Osteoconductive activity of the biomaterial was confirmed by the presence of osteoblasts in the biopsy specimens. Absence of a chronic inflammatory response or foreign body reaction indicated biocompatibility. Expression of osteoblast markers was detected in the newly formed tissue. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated that castor oil polyurethane polymer with calcium carbonate could be an acceptable compound for use as a bone substitute in horses with fractures in which bone filling is necessary.
目的 评估蓖麻油聚氨酯聚合物与碳酸钙用于马第三掌骨背侧单皮质截骨术的疗效。
动物 6 匹成年马。
方法 对每匹马的双侧第三掌骨背侧进行单皮质截骨术。将含有碳酸钙的蓖麻油（蓖麻）聚氨酯聚合物植入一侧肢体的截骨处，对侧肢体的截骨处不填充作为对照样本。120 天后对截骨部位进行组织学评估。从骨与聚合物的界面或骨与新形成组织的界面获取活检标本；标本经处理后，使用光学显微镜、免疫组织化学分析、组织化学分析和透射电子显微镜进行组织形态计量学评估。
结果 活检标本中存在成骨细胞，证实了该生物材料的骨传导活性。未出现慢性炎症反应或异物反应，表明具有生物相容性。在新形成的组织中检测到成骨细胞标志物的表达。
结论及临床意义 结果表明，含有碳酸钙的蓖麻油聚氨酯聚合物可作为一种可接受的化合物，用于需要骨填充的马骨折的骨替代物。
