Department of Chemistry and Molecular Physics, Institute of Chemistry of São Carlos, University of São Paulo, Brazil.
J Biomed Mater Res A. 2014 Feb;102(2):324-31. doi: 10.1002/jbm.a.34696. Epub 2013 May 14.
The aim of this study was to evaluate the modulation of matrix metalloproteinase-2 (MMP-2) and -9 (MMP-9) expression in newly formed bone tissue at the interface between implants derived from castor oil (Ricinus communis) polymer and the tibia medullary canal. Forty-four rabbits were assigned to either Group 1 (n = 12; control) or Group 2 (n = 30), which had the tibial medullary canals reamed bilaterally and filled with polymer. CT scans showed no space between the material surface and the bone at the implant/bone marrow interface, and the density of the tissues at this interface was similar to the density measured of other regions of the bone. At 90 days postimplantation, the interface with the polymer presented a thick layer of newly formed bone tissue rich in osteocytes. This tissue exhibited ongoing maturation at 120 and 150 days postimplantation. Overall, bone remodeling process was accompanied by positive modulation of MMP-2 and low MMP-9 expression. Differently, in control group, the internal surface close to the medullary canal was lined by osteoblasts, followed by a bone tissue zone with few lacunae filled with osteocytes. Maturation of the tissue of the medullary internal surface occurred in the inner region, with the bone being nonlamellar.
本研究旨在评估油基聚合物衍生植入物与胫骨骨髓腔界面处新形成的骨组织中基质金属蛋白酶-2（MMP-2）和-9（MMP-9）表达的调节。44 只兔子被分配到第 1 组（n=12；对照组）或第 2 组（n=30），第 2 组双侧胫骨骨髓腔扩髓并用聚合物填充。CT 扫描显示在植入物/骨髓界面处，材料表面与骨之间没有间隙，并且该界面处组织的密度与骨的其他区域测量的密度相似。在植入后 90 天，与聚合物的界面呈现出富含骨细胞的厚层新形成的骨组织。在植入后 120 和 150 天，该组织仍在不断成熟。总的来说，骨重塑过程伴随着 MMP-2 的积极调节和 MMP-9 的低表达。相比之下，在对照组中，靠近骨髓腔的内部表面被成骨细胞排列，随后是一个有几个充满骨细胞的腔隙的骨组织区。骨髓内部表面的组织成熟发生在内区，骨是非板层状的。
