Xia Fan, Cai Yu-Min, Chen Dong-Xia, Qiu Li-Hong
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, PR China.
Int J Syst Evol Microbiol. 2017 Oct;67(10):4260-4265. doi: 10.1099/ijsem.0.002300. Epub 2017 Sep 25.
Two strains, 4G-K17 and 4G-K15, were isolated from forest soil from the Dinghushan Biosphere Reserve, Guangdong Province, PR China (112° 31' E 23° 10' N). The cells of the two strains were Gram-stain-negative, aerobic and non-motile short rods that multiplied by binary division. Strains 4G-K17 and 4G-K15 were obligately acidophilic, mesophilic bacteria capable of growth at pH 3.0-7.0 (optimum 4.0-5.5 and 3.5-5.5, respectively), temperature 12-42 °C (optimum 28 °C) and NaCl concentrations from 02.5 % and 03.0 % (w/v), respectively. They had a 16S rRNA gene sequence similarity of 99.2 % and showed the highest similarities of 97.1 and 97.4 % to Edaphobacter aggregans Wbg-1, respectively, which indicated that these two isolates belonged to a novel species of the genus Edaphobacter in subdivision 1 in the family Acidobacteriaceae. The DNA G+C contents of strains 4G-K17 and 4G-K15 were 57.6 and 57.2 %, respectively. They had similar fatty acid profiles, with the major (>10 %) fatty acid profile comprising iso-C15 : 0, C16 : 0 and summed feature 3 (iso-C16 : 1ω7c and/or C16 : 1ω6c), and the major polar lipid profile comprising phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, unidentified aminophospholipids and unidentified phospholipids. All physiological, phylogenetic and chemotaxonomic data suggest that strains 4G-K17 and 4G-K15 belong to a new species of the genus Edaphobacter, for which the name Edaphobacter acidisoli sp. nov. is proposed, with 4G-K17 (=CGMCC 1.15447=LMG 29212) as the type strain.
从中国广东省鼎湖山生物圈保护区的森林土壤中分离出两株菌株，4G-K17和4G-K15（东经112°31′，北纬23°10′）。这两株菌株的细胞革兰氏染色阴性，好氧，不运动，为短杆菌，通过二分分裂进行繁殖。菌株4G-K17和4G-K15是专性嗜酸、嗜温细菌，能够在pH 3.0 - 7.0（最适pH分别为4.0 - 5.5和3.5 - 5.5）、温度12 - 42°C（最适温度28°C）以及NaCl浓度分别为02.5%和03.0%（w/v）的条件下生长。它们的16S rRNA基因序列相似性为99.2%，与聚集体土杆菌Wbg-1的相似性最高，分别为97.1%和97.4%，这表明这两株分离株属于酸杆菌科1亚群中土杆菌属的一个新物种。菌株4G-K17和4G-K15的DNA G+C含量分别为57.6%和57.2%。它们具有相似的脂肪酸谱，主要（>10%）脂肪酸谱包括异-C15:0、C16:0和总和特征3（异-C16:1ω7c和/或C16:1ω6c），主要极性脂质谱包括磷脂酰乙醇胺、二磷脂酰甘油、磷脂酰甘油、未鉴定的氨基磷脂和未鉴定的磷脂。所有生理、系统发育和化学分类学数据表明，菌株4G-K17和4G-K15属于土杆菌属的一个新物种，为此提出新名称嗜酸土杆菌（Edaphobacter acidisoli sp. nov.），以4G-K17（=CGMCC 1.15447=LMG 29212）作为模式菌株。
