Ou-Yang Tian-Na, Xia Fan, Qiu Li-Hong
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, PR China.
Int J Syst Evol Microbiol. 2018 Jul;68(7):2364-2369. doi: 10.1099/ijsem.0.002846. Epub 2018 Jun 6.
A bacterial strain, designated 4GSKX, isolated from the forest soil of Dinghushan Biosphere Reserve, Guangdong Province, PR China (112° 31' E 23° 10' N), is proposed as a novel species of the genus Acidicapsa. Cells of strain 4GSKX were aerobic, non-motile, Gram-stain-negative short rods that multiplied by binary division. The strain grew at 12-37 °C (optimum, 25-30 °C), pH 4.0-6.5 (optimum, pH 4.5-5.0) and NaCl concentrations of 0-1.0 % (w/v; optimum, 0 %). Strain 4GSKX utilized various carbon sources as growth substrates, including both sugars and amino acids. The major fatty acids (>10 %) were iso-C15 : 0 (48.8 %) and iso-C17 : 1ω9c/C16 : 0 10-methyl (14.7 %). The major polar lipids were phosphatidylethanolamine, an unidentified glycolipid, three unidentified phospholipids and two unidentified aminophospholipids. The only quinone detected was MK-8 and the DNA G+C content was 52.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 4GSKX belongs to the genus Acidicapsa in the family Acidobacteriaceae in subdivision 1 of the phylum Acidobacteria, with the highest similarity of 97.1 % to Acidicapsa ligni WH120. Based on all phenotypic, chemotaxonomic and phylogenetic data obtained, it is proposed as a novel species of genus Acidicapsa, for which the name Acidicapsa dinghuensis sp. nov. is proposed, with 4GSKX (=CGMCC 1.15449=LMG 29213) as the type strain.
从中国广东省鼎湖山生物圈保护区森林土壤（东经112°31′，北纬23°10′）分离出的一株细菌，编号为4GSKX，被提议作为嗜酸菌属的一个新物种。4GSKX菌株的细胞为需氧型、无运动性、革兰氏染色阴性短杆菌，通过二分分裂繁殖。该菌株在12 - 37 °C（最适温度为25 - 30 °C）、pH 4.0 - 6.5（最适pH为4.5 - 5.0）和NaCl浓度0 - 1.0 %（w/v；最适浓度为0 %）的条件下生长。4GSKX菌株利用多种碳源作为生长底物，包括糖类和氨基酸。主要脂肪酸（>10 %）为异-C15 : 0（48.8 %）和异-C17 : 1ω9c/C16 : 0 10-甲基（14.7 %）。主要极性脂为磷脂酰乙醇胺、一种未鉴定的糖脂、三种未鉴定的磷脂和两种未鉴定的氨基磷脂。检测到的唯一醌为MK-8，DNA G+C含量为52.8 mol%。基于16S rRNA基因序列的系统发育分析表明，4GSKX菌株属于酸杆菌门第1亚纲酸杆菌科嗜酸菌属，与嗜酸木杆菌WH120的相似度最高，为97.1 %。根据所获得的所有表型、化学分类和系统发育数据，提议将其作为嗜酸菌属的一个新物种，命名为鼎湖嗜酸菌（Acidicapsa dinghuensis sp. nov.），模式菌株为4GSKX（=CGMCC 1.15449=LMG 29213）。
