Weissenbacher-Lang Christiane, Nedorost Nora, Knecht Christian, Hennig-Pauka Isabel, Huber Mathias, Voglmayr Thomas, Weissenböck Herbert
Institute of Pathology and Forensic Veterinary Medicine, Department of Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria.
University Clinic for Swine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Vienna, Austria.
PLoS One. 2017 Sep 25;12(9):e0185387. doi: 10.1371/journal.pone.0185387. eCollection 2017.
Pneumocystis carinii f. sp. suis (PCS) nucleic acid and antibody profiles on two Austrian-farrow-to-finish farms were investigated. Furthermore, associations with other respiratory pathogens were evaluated. Respiratory specimen and sera from pigs of five age classes between the 1st week and the 3rd month of life as well as samples from sows were analyzed. On Farm A, PCS infection occurred early in life. The suckling piglets were already infected in the 1st week of life and the pigs remained positive until the 3rd month of life. On Farm B, pigs were infected later, between 3 and 4 months of age. The maximum PCS nucleic acid load on Farm A was 8.3 log10 genome copies/mL BALF, whereas on Farm B the PCS burden was significantly lower, with 4.0 log10 genome copies/mL BALF. Anti-PCS antibodies were detected in sows, as maternal antibodies in suckling piglets and as an immunological reaction to infection. On both farms, PCS infection was accompanied by several co-infections. On Farm A, there were concurrent infections with PRRSV, a virulent strain of Haemophilus parasuis, and Mycoplasma hyopneumoniae. On Farm B, PCS was accompanied by infections with swine influenza virus, Mycoplasma hyopneumoniae, and a non-virulent strain of Haemophilus parasuis. The results clearly show that the PCS profiles can vary between farms. Younger pigs may be more susceptible as they had higher PCS burdens. It is possible that PCS may contribute to a respiratory disease in pigs and further investigation of its potential role is warranted.
对奥地利两个从仔猪到育肥猪的养殖场中的猪卡氏肺孢子虫猪亚种（PCS）核酸和抗体谱进行了调查。此外，还评估了其与其他呼吸道病原体的关联。分析了1周龄至3月龄五个年龄组猪的呼吸道标本和血清以及母猪的样本。在农场A，PCS感染在生命早期发生。哺乳仔猪在出生后第1周就已感染，并且这些猪直到3月龄时仍呈阳性。在农场B，猪在3至4月龄时感染较晚。农场A的最大PCS核酸载量为8.3 log10基因组拷贝/毫升支气管肺泡灌洗液（BALF），而农场B的PCS负荷明显较低，为4.0 log10基因组拷贝/毫升BALF。在母猪中检测到抗PCS抗体，在哺乳仔猪中作为母源抗体，以及作为对感染的免疫反应。在两个农场中，PCS感染都伴有几种合并感染。在农场A，同时感染了猪繁殖与呼吸综合征病毒（PRRSV）、致病性副猪嗜血杆菌菌株和猪肺炎支原体。在农场B，PCS伴有猪流感病毒、猪肺炎支原体和非致病性副猪嗜血杆菌菌株的感染。结果清楚地表明，不同农场之间的PCS谱可能有所不同。较年轻的猪可能更易感染，因为它们的PCS负荷较高。PCS有可能导致猪的呼吸道疾病，因此有必要进一步研究其潜在作用。
