使用亲水作用色谱-电喷雾电离-离子阱-飞行时间质谱系统对六种坚果中的磷脂谱进行表征。
Characterization of phospholipid profiles in six kinds of nut using HILIC-ESI-IT-TOF-MS system.
作者信息
Song Shuang, Cheong Ling-Zhi, Wang Hui, Man Qing-Qing, Pang Shao-Jie, Li Yue-Qi, Ren Biao, Wang Zhu, Zhang Jian
机构信息
National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Department of Food Science, School of Marine Science, Ningbo University, Ningbo, China.
出版信息
Food Chem. 2018 Feb 1;240:1171-1178. doi: 10.1016/j.foodchem.2017.08.021. Epub 2017 Aug 4.
Nuts, a class of fatty natural food, are associated with a series of health benefit. Lipid, which is the most abundant nutrient in nuts, could be one of the major contributors to many beneficial effects. In previous studies, only the composition of fatty acids was investigated. To better understand the nutritional value of nuts, phospholipids (PLs), with widely confirmed bioactivities, should be profiled as well. In this work, a HILIC-ESI-IT-TOF-MS system is applied to characterize PL profiles in six kinds of nut, including almonds, cashews, pecans, pistachios, walnuts and peanuts. A total of 165 PL molecular species were characterized and quantified. The obtained results showed that pistachios, cashews and walnuts are favorable nuts with diverse and high content of PLs; peanuts consist of abundant PL species but lower concentration of PLs; almonds and pecans might not be the first choice for PLs with relatively low species abundance and content.
坚果是一类富含脂肪的天然食物，具有一系列健康益处。脂质是坚果中含量最丰富的营养成分，可能是许多有益作用的主要贡献者之一。在以往的研究中，仅对脂肪酸组成进行了研究。为了更好地了解坚果的营养价值，还应分析具有广泛证实的生物活性的磷脂（PLs）。在这项工作中，采用亲水相互作用液相色谱-电喷雾电离-离子阱-飞行时间质谱系统（HILIC-ESI-IT-TOF-MS）对六种坚果（包括杏仁、腰果、山核桃、开心果、核桃和花生）中的磷脂谱进行表征。共鉴定和定量了165种磷脂分子种类。所得结果表明，开心果、腰果和核桃是富含多种磷脂且含量高的优质坚果；花生含有丰富的磷脂种类，但磷脂浓度较低；杏仁和山核桃的磷脂种类丰度和含量相对较低，可能不是获取磷脂的首选。
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