植物乳杆菌ST8SH产生的细菌素与万古霉素、蜂胶或乙二胺四乙酸联合对控制单核细胞增生李斯特菌生物膜形成的作用
Combined effect of bacteriocin produced by Lactobacillus plantarum ST8SH and vancomycin, propolis or EDTA for controlling biofilm development by Listeria monocytogenes.
作者信息
Todorov Svetoslav D, de Paula Otávio A L, Camargo Anderson C, Lopes Danilo A, Nero Luís A
机构信息
Universidade Federal de Viçosa, Departamento de Veterinária, Campus UFV, 36570-900 Viçosa, MG, Brazil.
Universidade Federal de Viçosa, Departamento de Veterinária, Campus UFV, 36570-900 Viçosa, MG, Brazil.
出版信息
Rev Argent Microbiol. 2018 Jan-Mar;50(1):48-55. doi: 10.1016/j.ram.2017.04.011. Epub 2017 Sep 23.
The Listeria monocytogenes strains selected in the present study exhibited similar behavior in biofilm formation, independently of the tested conditions (bacteriocin from L. plantarum ST8SH, vancomycin, propolis (a natural antimicrobial product) and EDTA (chelating agent)), individual or in associations. The individual application of vancomycin had better inhibitory activity than that of propolis and EDTA; however, the association of the previously mentioned antimicrobial agents with bacteriocins resulted in better performance. However, when we compared the effects of vancomycin, propolis and EDTA, we could clearly observe that the combined application of bacteriocin and vancomycin was more effective than the combination of bacteriocin and propolis, and bacteriocin and EDTA. Considering the current need to reduce the use of antimicrobials and chemical substances in food processing, propolis can represent an alternative to improve the inhibitory effect of bacteriocins against L. monocytogenes biofilm formation, based on the obtained results. In general, high concentrations of bacteriocin produced by L. plantarum ST8SH were more effective in biofilm inhibition, and similar results were observed for vancomycin and propolis; however, all tested EDTA concentrations had similar effect on biofilm formation.
本研究中选择的单核细胞增生李斯特菌菌株在生物膜形成方面表现出相似的行为，与测试条件（植物乳杆菌ST8SH产生的细菌素、万古霉素、蜂胶（一种天然抗菌产品）和乙二胺四乙酸（螯合剂））无关，无论是单独使用还是联合使用。单独使用万古霉素的抑制活性比蜂胶和乙二胺四乙酸更好；然而，上述抗菌剂与细菌素联合使用时效果更佳。然而，当我们比较万古霉素、蜂胶和乙二胺四乙酸的效果时，可以清楚地观察到，细菌素与万古霉素联合使用比细菌素与蜂胶、细菌素与乙二胺四乙酸联合使用更有效。考虑到当前食品加工中减少抗菌剂和化学物质使用的需求，基于所得结果，蜂胶可作为一种替代物，用于提高细菌素对单核细胞增生李斯特菌生物膜形成的抑制作用。总体而言，植物乳杆菌ST8SH产生的高浓度细菌素对生物膜抑制更有效，万古霉素和蜂胶也观察到类似结果；然而，所有测试浓度的乙二胺四乙酸对生物膜形成的影响相似。
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