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[使用Reflotron系统进行猪和羊的实验室诊断]

[Use of the Reflotron system for laboratory diagnosis in swine and sheep].

作者信息

Bickhardt K, Meyer B

机构信息

Klinik für kleine Klauentiere und forensische Medizin, Tierärztlichen Hochschule Hannover.

出版信息

Tierarztl Prax. 1987;15(4):435-9.

PMID:2894725
Abstract

The "Reflotron" system (Boehringer Mannheim) was compared with standard laboratory methods for determination of blood values, e.g. haemoglobin, glucose, urea, ASAT/GOT and GGT in pigs and sheep. Various different commercial control sera as well as blood and plasma samples from pigs and sheep were used for evaluation of its precision. The day-to-day precision of all parameters was satisfactory. The accuracy of the values was tested in 56(48) blood samples of healthy and sick pigs (sheep) by using standard substances (glucose, urea) and by comparing them with values measured by standard laboratory methods. There were systematic differences between "Reflotron" and other values in the glucose- and enzyme-values, which can be corrected with the help of conversion factors. In pigs, the enzyme GGT can not be determined accurately with the "Reflotron" system.

摘要

将“Reflotron”系统(勃林格殷格翰公司)与用于测定猪和羊血液指标(如血红蛋白、葡萄糖、尿素、谷草转氨酶/天冬氨酸氨基转移酶和γ-谷氨酰转移酶)的标准实验室方法进行了比较。使用了各种不同的商业对照血清以及猪和羊的血液及血浆样本对其精密度进行评估。所有参数的日常精密度令人满意。通过使用标准物质(葡萄糖、尿素)并将其与标准实验室方法测得的值进行比较,在56份(48份)健康和患病猪(羊)的血液样本中测试了这些值的准确性。在葡萄糖和酶值方面,“Reflotron”系统与其他值之间存在系统性差异,可借助转换因子进行校正。在猪中,使用“Reflotron”系统无法准确测定酶γ-谷氨酰转移酶。

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