Ishii H, Watanabe Y, Okuno F, Takagi T, Munakata Y, Miura S, Shigeta Y, Tsuchiya M
Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.
Alcohol Clin Exp Res. 1988 Feb;12(1):111-5. doi: 10.1111/j.1530-0277.1988.tb00142.x.
It is a well-known phenomenon that serum gamma-glutamyltranspeptidase (gamma-GTP, EC 2.3.2.2.) activity is increased after chronic consumption of ethanol, and gamma-GTP has been, therefore, widely used as a sensitive marker for detection of alcoholism and its related liver disease. However, the precise mechanisms whereby the chronic ethanol consumption leads to an increase in serum gamma-GTP activity are not fully understood. In the present study, we investigated the relationship between the intestinal and serum gamma-GTP activities after chronic ethanol consumption both in rats and humans. Chronic ethanol feeding to rats resulted in a significant increase in serum gamma-GTP activity associated with a significant increment of the intestinal gamma-GTP activity. The histochemical staining of gamma-GTP in the mucosa of the small intestine of these animals demonstrated enhanced gamma-GTP activity at the microvilli of the brush border membrane, lamina propria of the mucosa, and endoplasmic reticulum of the intestinal epithelial cell. The augmented activity in the lamina propria was mainly localized at the submucosal lymphatics. Histology of the small intestine of human alcoholics was, more or less, similar to those observed in alcoholic rats. We further investigated the gamma-GTP activity in the mesenteric lymph using the animal model of lymphorrhea, and found that the gamma-GTP activity was increased by 83% when expressed per unit of lymph in the ethanol-fed rat, accompanied by a marked decrease of serum gamma-GTP activity, suggesting a close relationship between the serum and the intestinal gamma-GTP via the lymphatic channel.(ABSTRACT TRUNCATED AT 250 WORDS)
长期摄入乙醇后血清γ-谷氨酰转肽酶（γ-GTP，EC 2.3.2.2.）活性升高是一个众所周知的现象，因此γ-GTP已被广泛用作检测酒精中毒及其相关肝脏疾病的敏感标志物。然而，长期摄入乙醇导致血清γ-GTP活性增加的确切机制尚未完全明确。在本研究中，我们调查了大鼠和人类长期摄入乙醇后肠道γ-GTP活性与血清γ-GTP活性之间的关系。给大鼠长期喂食乙醇导致血清γ-GTP活性显著增加，同时肠道γ-GTP活性也显著升高。对这些动物小肠黏膜进行γ-GTP组织化学染色显示，在刷状缘膜微绒毛、黏膜固有层和肠上皮细胞内质网处γ-GTP活性增强。固有层中增强的活性主要定位于黏膜下淋巴管。人类酗酒者小肠的组织学表现或多或少与酒精性大鼠相似。我们使用淋巴溢动物模型进一步研究了肠系膜淋巴中的γ-GTP活性，发现以每单位淋巴计算，乙醇喂养大鼠的γ-GTP活性增加了83%，同时血清γ-GTP活性显著降低，这表明血清γ-GTP与肠道γ-GTP通过淋巴通道密切相关。（摘要截选至250词）
