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基于癌症基因组图谱（TCGA）的网络分析揭示了结肠癌中的关键基因。

Network analysis based on TCGA reveals hub genes in colon cancer.

作者信息

Wu Fenzan, Yuan Guoping, Chen Junjie, Wang Chengzu

机构信息

Science and Education Division, Cixi Affiliated Hospital of Wenzhou Medical University, Zhejiang, China.

Clinical Laboratory, Cixi Affiliated Hospital of Wenzhou Medical University, Zhejiang, China.

出版信息

Contemp Oncol (Pozn). 2017;21(2):136-144. doi: 10.5114/wo.2017.68622. Epub 2017 Jun 30.

DOI:10.5114/wo.2017.68622
PMID:28947883
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5611503/
Abstract

Colorectal cancer (CRC) is the third most widespread cancer in the world. Although many advances have been made in molecular biology, novel approaches are still required to reveal molecular mechanisms for the diagnosis and therapy of colon cancer. In this study, we aimed to determine and analyse the hub genes of CRC. First, we explored the mRNA and microRNA (miRNA) expression profiles of colon carcinoma, then we screened target genes of differentially expressed miRNAs and obtained the intersection between differently expressed genes and target genes. Gene Ontology (GO) classification and KEGG pathway analysis of differently expressed genes were performed, and gene-miRNA and TF-gene-miRNA networks were constructed to identify hub genes, miRNAs, and TFs. In total, 3436 significant differentially expressed genes (1709 upregulated and 1727 downregulated) and 216 differentially expressed miRNAs (99 upregulated and 117 downregulated) were identified in colon cancer. These differentially expressed genes were significantly enriched in GO terms and KEGG pathways, such as cell proliferation, cell adhesion, and cytokine-cytokine receptor interaction signalling pathways. , , and so on were located in the central hub of the co-expression network. mir-34a, and LEF1 were located in the central hub of the network of TF-gene-miRNA. These findings increase our understanding of the molecular mechanisms of colon cancer and will aid in identifying potential targets for diagnostic and therapeutic usage.

摘要

结直肠癌（CRC）是全球第三大常见癌症。尽管分子生物学已取得诸多进展，但仍需要新方法来揭示结肠癌诊断和治疗的分子机制。在本研究中，我们旨在确定并分析CRC的核心基因。首先，我们探究了结肠癌的mRNA和微小RNA（miRNA）表达谱，接着筛选差异表达miRNA的靶基因，并获得差异表达基因与靶基因的交集。对差异表达基因进行基因本体论（GO）分类和KEGG通路分析，并构建基因-miRNA和转录因子-基因-miRNA网络以鉴定核心基因、miRNA和转录因子。在结肠癌中总共鉴定出3436个显著差异表达基因（1709个上调和1727个下调）和216个差异表达miRNA（99个上调和117个下调）。这些差异表达基因在GO术语和KEGG通路中显著富集，如细胞增殖、细胞黏附以及细胞因子-细胞因子受体相互作用信号通路等。 等位于共表达网络的中心枢纽。mir-34a和LEF1位于转录因子-基因-miRNA网络的中心枢纽。这些发现增进了我们对结肠癌分子机制的理解，并将有助于确定诊断和治疗用途的潜在靶点。

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