Li Chunyan, Wang Yulin, Lu Shuming, Zhang Zhuqing, Meng Hua, Liang Lina, Zhang Yan, Song Bo
Department of Gastroenterology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China.
Department of General Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China.
Mol Med Rep. 2015 Nov;12(5):7072-8. doi: 10.3892/mmr.2015.4263. Epub 2015 Aug 28.
The microRNA (miRNA), miR‑34a is significant in colon cancer progression. In the present study, the role of miR‑34a in colon cancer cell proliferation and metastasis was investigated. It was found that the expression of miR‑34a in colon cancer tissues and cell lines was lower when compared with that of normal tissues and cells. Further research demonstrated that miR‑34a inhibited cell proliferation, induced G1 phase arrest, and suppressed metastasis and epithelial mesenchymal transition in colon cancer cells. Bioinformatic prediction indicated that platelet‑derived growth factor receptor α (PDGFRA) was a potential target gene of miR‑34a and a luciferase assay identified that PDGFRA was a novel direct target gene of miR‑34a. In addition, assays of western blot analyses and quantitative reverse‑transcription polymerase chain reaction confirmed that miR‑34a decreased PDGFRA mRNA expression and protein levels in colon cancer cells. Assessment of cellular function indicated that miR‑34a inhibited colon cancer progression via PDGFRA. These findings demonstrate that miR‑34a may act as a negative regulator in colon cancer by targeting PDGFRA.
微小RNA（miRNA），即miR-34a在结肠癌进展中具有重要意义。在本研究中，对miR-34a在结肠癌细胞增殖和转移中的作用进行了研究。结果发现，与正常组织和细胞相比，结肠癌组织和细胞系中miR-34a的表达较低。进一步研究表明，miR-34a抑制结肠癌细胞增殖，诱导G1期阻滞，并抑制转移和上皮-间质转化。生物信息学预测表明，血小板衍生生长因子受体α（PDGFRA）是miR-34a的潜在靶基因，荧光素酶报告基因检测确定PDGFRA是miR-34a的一个新的直接靶基因。此外，蛋白质印迹分析和定量逆转录聚合酶链反应检测证实，miR-34a降低了结肠癌细胞中PDGFRA的mRNA表达和蛋白水平。细胞功能评估表明，miR-34a通过PDGFRA抑制结肠癌进展。这些发现表明，miR-34a可能通过靶向PDGFRA在结肠癌中发挥负调节作用。
