Kong Weilong, Yang Shaozong, Wang Yulu, Bendahmane Mohammed, Fu Xiaopeng
College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Key Laboratory of Horticultural Plant Biology, Ministry of Education, Wuhan, Hubei, China.
INRA-CNRS-Lyon1-ENS, Laboratoire Reproduction et Developpement des Plantes, Ecole Normale Supérieure Lyon, France.
PeerJ. 2017 Sep 19;5:e3747. doi: 10.7717/peerj.3747. eCollection 2017.
Aquaporins (AQPs) are essential channel proteins that execute multi-functions throughout plant growth and development, including water transport, uncharged solutes uptake, stress response, and so on. Here, we report the first genome-wide identification and characterization () genes in sugar beet () an important crop widely cultivated for feed, for sugar production and for bioethanol production. Twenty-eight sugar beet () were identified and assigned into five subfamilies based on phylogenetic analyses: seven of plasma membrane (PIPs), eight of tonoplast (TIPs), nine of NOD26-like (NIPs), three of small basic (SIPs), and one of x-intrinsic proteins (XIPs). genes unevenly mapped on all chromosomes, except on chromosome 4. Gene structure and motifs analyses revealed that have conserved exon-intron organization and that they exhibit conserved motifs within each subfamily. Prediction of BvAQPs functions, based on key protein domains conservation, showed a remarkable difference in substrate specificity among the five subfamilies. Analyses of expression, by mean of RNA-seq, in different plant organs and in response to various abiotic stresses revealed that they were ubiquitously expressed and that their expression was induced by heat and salt stresses. These results provide a reference base to address further the function of sugar beet aquaporins and to explore future applications for plants growth and development improvements as well as in response to environmental stresses.
水通道蛋白（AQPs）是一类重要的通道蛋白，在植物生长发育过程中执行多种功能，包括水分运输、不带电荷溶质的吸收、应激反应等。在此，我们首次对甜菜（一种广泛种植用于饲料、制糖和生产生物乙醇的重要作物）中的水通道蛋白基因进行了全基因组鉴定和特征分析。基于系统发育分析，共鉴定出28个甜菜水通道蛋白基因，并将其分为五个亚家族：7个质膜水通道蛋白（PIPs）、8个液泡膜水通道蛋白（TIPs）、9个NOD26样水通道蛋白（NIPs）、3个小碱性水通道蛋白（SIPs）和1个X-内在蛋白（XIPs）。水通道蛋白基因不均匀地分布在除4号染色体外的所有染色体上。基因结构和基序分析表明，甜菜水通道蛋白具有保守的外显子-内含子组织，并且在每个亚家族中都表现出保守的基序。基于关键蛋白结构域保守性对甜菜水通道蛋白功能进行的预测表明，五个亚家族在底物特异性上存在显著差异。通过RNA测序分析甜菜水通道蛋白在不同植物器官中的表达以及对各种非生物胁迫的响应，结果表明它们在植物中普遍表达，并且其表达受高温和盐胁迫诱导。这些结果为进一步研究甜菜水通道蛋白的功能以及探索其在改善植物生长发育和应对环境胁迫方面的未来应用提供了参考依据。
