Chen Caihui, Zheng Yongjie, Liu Sian, Zhong Yongda, Wu Yanfang, Li Jiang, Xu Li-An, Xu Meng
Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, Jiangsu, China.
Camphor Engineering Technology Research Center for State Forestry Administration, Jiangxi Academy of Forestry, Nanchang, Jiangxi, China.
PeerJ. 2017 Sep 18;5:e3820. doi: 10.7717/peerj.3820. eCollection 2017.
, a member of the family, is a valuable aromatic and timber tree that is indigenous to the south of China and Japan. All parts of have secretory cells containing different volatile chemical compounds that are utilized as herbal medicines and essential oils. Here, we reported the complete sequencing of the chloroplast genome of using illumina technology. The chloroplast genome of is 152,570 bp in length and characterized by a relatively conserved quadripartite structure containing a large single copy region of 93,705 bp, a small single copy region of 19,093 bp and two inverted repeat (IR) regions of 19,886 bp. Overall, the genome contained 123 coding regions, of which 15 were repeated in the IR regions. An analysis of chloroplast sequence divergence revealed that the small single copy region was highly variable among the different genera in the family. A total of 40 repeat structures and 83 simple sequence repeats were detected in both the coding and non-coding regions. A phylogenetic analysis indicated that is most closely related to , both being members of , which forms a sister group to . The complete sequence of the chloroplast of will aid in in-depth taxonomical studies of the family in the future. The genetic sequence information will also have valuable applications for chloroplast genetic engineering.
[植物名称]是[植物所属科名]科的一员,是一种珍贵的芳香和用材树种,原产于中国南部和日本。[植物名称]的所有部分都含有分泌细胞,这些细胞含有不同的挥发性化合物,可作为草药和精油使用。在此,我们报道了利用Illumina技术对[植物名称]叶绿体基因组进行的全序列测定。[植物名称]的叶绿体基因组长度为152,570 bp,其特征是具有相对保守的四分体结构,包含一个93,705 bp的大单拷贝区域、一个19,093 bp的小单拷贝区域和两个19,886 bp的反向重复(IR)区域。总体而言,该基因组包含123个编码区,其中15个在IR区域重复。叶绿体序列差异分析表明,小单拷贝区域在[植物所属科名]科的不同属之间高度可变。在编码区和非编码区共检测到40个重复结构和83个简单序列重复。系统发育分析表明,[植物名称]与[另一植物名称]关系最为密切,它们都是[植物所属科名]科的成员,共同构成了[某一植物类群]的姐妹群。[植物名称]叶绿体的完整序列将有助于未来对[植物所属科名]科进行深入的分类学研究。该遗传序列信息也将在叶绿体基因工程中具有重要应用价值。