成纤维细胞生长因子 22 通过白细胞介素-1β 成为一种新型的抑郁症调节剂。
Fibroblast growth factor 22 is a novel modulator of depression through interleukin-1β.
机构信息
Affiliated Hospital of Jiangsu University, Zhenjiang, China.
The Forth Affiliated Hospital of Jiangsu University, Zhenjiang, China.
出版信息
CNS Neurosci Ther. 2017 Nov;23(11):907-916. doi: 10.1111/cns.12760. Epub 2017 Sep 25.
BACKGROUND AND AIMS
Emerging evidence shows that fibroblast growth factor 22 (FGF22) plays a critical role in the etiology of depression. However, the molecular mechanisms of FGF22 are not fully comprehended. Here, the effect of FGF22 in depression and its relationship with interleukin-1β (IL-1β) were investigated in clinical, animal, and cell experiments.
METHODS
Serum from depressive patients was collected, and the levels of FGF22 and IL-1β were analyzed by ELISA. The chronic unpredictable mild stress (CUMS) model was established, and primary hippocampal neuronal cells were cultured to examine changes in FGF22 and IL-1β levels in rat hippocampus.
RESULTS
The results revealed a negative correlation between serum FGF22 levels and serum IL-1β levels. The expression of IL-1β in the CUMS rat hippocampus decreased, and the apoptosis of hippocampal cells improved after the injection of lentiviral vector-mediated FGF22 (LV-FGF22). Further tests in primary hippocampal neuronal cells also showed a reduction in IL-1β and the cell apoptosis rate after treatment with FGF22.
CONCLUSION
In conclusion, the results revealed that FGF22 plays a role in alleviating depression, which may be mediated by reduced expression of IL-1β.
背景与目的
新出现的证据表明，成纤维细胞生长因子 22（FGF22）在抑郁症的发病机制中起着关键作用。然而，FGF22 的分子机制尚未完全被理解。本研究通过临床、动物和细胞实验，探讨了 FGF22 在抑郁症中的作用及其与白细胞介素-1β（IL-1β）的关系。
方法
收集抑郁症患者的血清，通过 ELISA 分析 FGF22 和 IL-1β的水平。建立慢性不可预测轻度应激（CUMS）模型，培养原代海马神经元细胞，观察大鼠海马中 FGF22 和 IL-1β水平的变化。
结果
结果显示，血清 FGF22 水平与血清 IL-1β水平呈负相关。CUMS 大鼠海马中 IL-1β的表达减少，注射慢病毒载体介导的 FGF22（LV-FGF22）后海马细胞凋亡改善。在原代海马神经元细胞中的进一步测试也表明，FGF22 处理后 IL-1β和细胞凋亡率降低。
结论
总之，研究结果表明，FGF22 在缓解抑郁症中发挥作用，其机制可能是通过降低 IL-1β的表达来介导的。
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