Qin Xiao-Li, Sun Hui-Yuan, Yang Wu, Li Yong-Jun, Zheng Lin, Liu Ting, Huang Yong
Guizhou Provincial Key Laboratory of Pharmaceutics, Guiyang 550004, China.
School of Pharmacy, Guizhou Medical University, Guiyang 550004, China.
Zhongguo Zhong Yao Za Zhi. 2017 Jan;42(2):357-362. doi: 10.19540/j.cnki.cjcmm.20161222.018.
To investigate the metabolism of quercitrin in rat intestinal flora and possible biological pathways, laying the foundation for the metabolic mechanism of traditional Chinese medicine glycosides ingredients. UPLC-Q-TOF-MS/MS method was established to detect the quercitrin and its metabolites with 0.1% formic acid solution(A)-0.1% formic acid acetonitrile(B) as the mobile phase for gradient elution at a flow rate of 0.3 mL•min⁻¹. Electrospray negative ion mode was applied to analyze the metabolites of quercitrin in rat intestinal flora. Metabolite ToolsTM, mass defect filter(MDF) and other technologies were used to screen, analyze the metabolites and infer the chemical formula of the metabolites. The results showed that quercitrin would have degalactoside, deoxygenation and acetylation reactions, and the aglycone quercetin resulted from degalactoside would have further reactions such as hydroxylation, deoxygenation, reduction, and ring opening to achieve deoxygenation metabolite kaempferol, C2-C3 double bonds hydrogenation and reduction product taxifolin, and degalactoside product quercetin. The research results showed that quercitrin can be metabolized by rat intestinal flora, which could increase their hydrophobicity and chemical diversity.
为研究槲皮苷在大鼠肠道菌群中的代谢情况及可能的生物学途径，为中药苷类成分的代谢机制奠定基础。建立超高效液相色谱-四极杆-飞行时间串联质谱（UPLC-Q-TOF-MS/MS）法，以0.1%甲酸溶液（A）-0.1%甲酸乙腈（B）为流动相进行梯度洗脱，流速为0.3 mL•min⁻¹，检测槲皮苷及其代谢产物。采用电喷雾负离子模式分析大鼠肠道菌群中槲皮苷的代谢产物。运用Metabolite ToolsTM、质量亏损过滤（MDF）等技术对代谢产物进行筛选、分析并推断代谢产物的化学式。结果表明，槲皮苷会发生去半乳糖苷、脱氧和乙酰化反应，去半乳糖苷产生的苷元槲皮素还会进一步发生羟基化、脱氧、还原和开环等反应，从而得到脱氧代谢产物山奈酚、C2-C3双键氢化还原产物紫杉叶素以及去半乳糖苷产物槲皮素。研究结果表明，槲皮苷可被大鼠肠道菌群代谢，这会增加其疏水性和化学多样性。
