a Departamento de Biotecnologia, Genética e Biologia Celular , Universidade Estadual de Maringá , Maringá , Brazil.
b Centro de Energia Nuclear na Agricultura (CENA) , Universidade de São Paulo (USP) , Piracicaba , Brazil.
Nat Prod Res. 2018 Dec;32(23):2783-2790. doi: 10.1080/14786419.2017.1380011. Epub 2017 Sep 26.
In the present study, biological activity and chemical composition of two crude extracts of endophytic fungal strains of genera isolated from two species of aquatic macrophytes: (Kunth) and (Mart.) were investigated. The nuclear magnetic resonance and mass spectrometry data provided the identification of three main compounds: curvulin (), spirostaphylotrichin R () and U (). The fragmentation mechanism of the precursor ions towards collision induced dissociation (CID) tandem mass spectrometry experiment (MS/MS) is also proposed. Furthermore, biological screening of the crude extracts displayed antileishmanial activity with IC values ranging from 70-84.2 μg.mL.
本研究调查了从两种水生植物（Kunth）和（Mart.）中分离出的内生真菌菌株的两种粗提物的生物活性和化学成分。核磁共振和质谱数据鉴定了三种主要化合物：卷曲霉素（）、螺旋甾醇三烯 R（）和 U（）。还提出了前体离子在碰撞诱导解离（CID）串联质谱实验（MS/MS）中碎裂的机制。此外，粗提取物的生物筛选显示出抗利什曼原虫活性，IC 值范围为 70-84.2μg.mL。
