Institute of Coastal Environmental Pollution Control, Key Lab of Marine Environmental Science and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China; College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550081, China.
Bioresour Technol. 2018 Jan;247:273-281. doi: 10.1016/j.biortech.2017.09.049. Epub 2017 Sep 8.
Harmful algal blooms (HABs) have become global environmental issues, and the demand for alternative algaecides is urgent. Pyrolytic vinegars (PVs) were pyrolyzed from giant reed at 300-600°C to investigate the underlying mechanisms of their inhibitory effect on the red tide dinoflagellate Karenia brevis by sub-chronic toxicity experiments. The major components of PVs were acetic acid, phenols, aldehyde, ketone, and esters. The 96h median effective concentration (96h-EC) values of PVs were 0.65-1.08mLL, and PV300 showed the strongest inhibitory effect. The increased contents of reactive oxygen species (ROS) and malondialdehyde, antioxidant enzymes activities indicated that K. brevis cells were suffering from oxidative stress, leading to lipid oxidation and cell structure damage. The sites of ROS accumulation in the treated cells were chloroplasts and mitochondria. These results suggest the suitability of PVs as potential algaecides for HAB control, and also provide a new direction for biomass valorization.
有害藻华（HAB）已成为全球性环境问题，对替代杀藻剂的需求迫在眉睫。本研究以巨菌草为原料，在 300-600°C 下热解制备热解醋，通过亚慢性毒性实验探究其对赤潮甲藻凯伦藻的抑制作用机制。热解醋的主要成分是乙酸、酚类、醛类、酮类和酯类。热解醋对凯伦藻的 96 小时半数有效浓度（96h-EC）值为 0.65-1.08mLL，其中 PV300 的抑制效果最强。活性氧（ROS）和丙二醛含量的增加，抗氧化酶活性的提高表明凯伦藻细胞受到氧化应激，导致脂质氧化和细胞结构损伤。ROS 在处理细胞中的积累部位是叶绿体和线粒体。这些结果表明热解醋具有作为控制有害藻华的潜在杀藻剂的适用性，也为生物质增值提供了新的方向。
