Department of Biochemistry and Molecular Biology III, School of Medicine, Complutense University of Madrid, Madrid, Spain.
Department of Physiology, School of Medicine, Complutense University of Madrid, Madrid, Spain.
J Nutr Biochem. 2017 Nov;49:133-140. doi: 10.1016/j.jnutbio.2017.07.011. Epub 2017 Jul 27.
It has been recently shown that xanthohumol, a flavonoid present in hops, possesses antioxidant, anti-inflammatory and chemopreventive properties. However, its role in the aging brain has not been addressed so far. Therefore, this study aimed to investigate the possible neuroprotective activity of xanthohumol against age-related inflammatory and apoptotic brain damage in male senescence-accelerated prone mice (SAMP8). Animals were divided into 4 groups: Untreated young mice, untreated old mice and old mice treated either with 1 mg kg day or 5 mg kg day xanthohumol. Young and old senescence accelerated resistant mice (SAMR1) were used as controls. After 30 days of treatment, animals were sacrificed and their brains were collected and immediately frozen in liquid nitrogen. mRNA (GFAP, TNF-α, IL-1β, AIF, BAD, BAX, XIAP, NAIP and Bcl-2) and protein (GFAP, TNF-α, IL-1β, AIF, BAD, BAX, BDNF, synaptophysin and synapsin) expressions were measured by RT-PCR and Western blotting, respectively. Significant increased levels of pro-inflammatory (TNF-α, IL-1β) and pro-apoptotic (AIF, BAD, BAX) markers were observed in both SAMP8 and SAMR1 old mice compared to young animals (P<.05) and also in SAMP8 untreated old mice compared to SAMR1 (P<.05). These alterations were significantly less evident in animals treated with both doses of xanthohumol (P<.05). Also, a reduced expression of synaptic markers was observed in old mice compared to young ones (P<.05) but it significantly recovered with 5 mg kg day xanthohumol treatment (P<.05). In conclusion, xanthohumol treatment modulated the inflammation and apoptosis of aged brains, exerting a protective effect on damage induced by aging.
最近有研究表明，啤酒花中的一种类黄酮——黄腐酚具有抗氧化、抗炎和化学预防特性。然而，其在衰老大脑中的作用尚未得到解决。因此，本研究旨在探讨黄腐酚对雄性快速衰老品系小鼠（SAMP8）衰老相关炎症和凋亡性脑损伤的可能神经保护作用。动物分为 4 组：未处理的年轻小鼠组、未处理的老年小鼠组和分别用 1mg/kg 天和 5mg/kg 天黄腐酚处理的老年小鼠组。使用未处理的快速衰老抵抗品系小鼠（SAMR1）作为对照。治疗 30 天后，处死动物并采集其大脑，立即在液氮中冷冻。通过 RT-PCR 和 Western blot 分别测量 mRNA（GFAP、TNF-α、IL-1β、AIF、BAD、BAX、XIAP、NAIP 和 Bcl-2）和蛋白（GFAP、TNF-α、IL-1β、AIF、BAD、BAX、BDNF、突触素和突触结合蛋白）的表达。与年轻动物相比，SAMP8 和 SAMR1 老年小鼠中促炎（TNF-α、IL-1β）和促凋亡（AIF、BAD、BAX）标志物的水平显著升高（P<.05），与 SAMR1 相比，SAMP8 未处理的老年小鼠中这些标志物的水平也显著升高（P<.05）。用两种剂量的黄腐酚处理后，这些变化明显不太明显（P<.05）。与年轻小鼠相比，老年小鼠的突触标志物表达减少（P<.05），但用 5mg/kg 天黄腐酚处理后明显恢复（P<.05）。总之，黄腐酚治疗调节了衰老大脑的炎症和凋亡，对衰老引起的损伤发挥了保护作用。
