Department of Biochemistry and Molecular Biology, School of Medicine, University Complutense of Madrid, Avda. Complutense s/n, 28040, Madrid, Spain.
Department Physiology, School of Medicine, Complutense University of Madrid, Avda. Complutense s/n, 28040, Madrid, Spain.
Eur J Nutr. 2019 Mar;58(2):653-663. doi: 10.1007/s00394-018-1657-6. Epub 2018 Mar 13.
Aging is associated with a deregulation of biological systems that lead to an increase in oxidative stress, inflammation, and apoptosis, among other effects. Xanthohumol is the main preylated chalcone present in hops (Humulus lupulus L.) whose antioxidant, anti-inflammatory and chemopreventive properties have been shown in recent years. In the present study, the possible protective effects of xanthohumol on liver alterations associated with aging were evaluated.
Male young and old senescence-accelerated prone mice (SAMP8), aged 2 and 10 months, respectively, were divided into four groups: non-treated young, non-treated old, old treated with 1 mg/kg/day xanthohumol, and old treated with 5 mg/kg/day xanthohumol. Male senescence-accelerated resistant mice (SAMR1) were used as controls. After 30 days of treatment, animals were sacrificed and livers were collected. mRNA (AIF, BAD, BAX, Bcl-2, eNOS, HO-1, IL-1β, NF-κB2, PCNA, sirtuin 1 and TNF-α) and protein expressions (BAD, BAX, AIF, caspase-3, Blc-2, eNOS, iNOS, TNF-α, IL1β, NF-κB2, and IL10) were measured by RT-PCR and Western blotting, respectively. Mean values were analyzed using ANOVA.
A significant increase in mRNA and protein levels of oxidative stress, pro-inflammatory and proliferative markers, as well as pro-apoptotic parameters was shown in old non-treated SAMP8 mice compared to the young SAMP8 group and SAMR1 mice. In general, age-related oxidative stress, inflammation and apoptosis were significantly decreased (p < 0.05) after XN treatment. In most cases, this effect was dose-dependent.
XN was shown to modulate inflammation, apoptosis, and oxidative stress in aged livers, exerting a protective effect in hepatic alterations.
衰老与生物系统的失调有关，导致氧化应激、炎症和细胞凋亡等增加。黄腐酚是啤酒花（Humulus lupulus L.）中主要的类姜黄素，近年来已显示其具有抗氧化、抗炎和化学预防特性。在本研究中，评估了黄腐酚对与衰老相关的肝脏改变的可能保护作用。
雄性年轻和年老快速衰老品系小鼠（SAMP8），分别为 2 个月和 10 个月大，分为四组：未治疗的年轻组、未治疗的老年组、老年组用 1mg/kg/天黄腐酚治疗组和老年组用 5mg/kg/天黄腐酚治疗组。雄性抗快速衰老品系小鼠（SAMR1）作为对照组。治疗 30 天后，处死动物并收集肝脏。通过 RT-PCR 和 Western blot 分别测量 mRNA（AIF、BAD、BAX、Bcl-2、eNOS、HO-1、IL-1β、NF-κB2、PCNA、Sirtuin 1 和 TNF-α）和蛋白质表达（BAD、BAX、AIF、caspase-3、Bcl-2、eNOS、iNOS、TNF-α、IL1β、NF-κB2 和 IL10）。使用方差分析分析平均值。
与年轻的 SAMP8 组和 SAMR1 组相比，未经治疗的老年 SAMP8 小鼠的氧化应激、促炎和增殖标志物以及促凋亡参数的 mRNA 和蛋白质水平显著增加。一般来说，XN 治疗后，与年龄相关的氧化应激、炎症和细胞凋亡显著降低（p<0.05）。在大多数情况下，这种作用是剂量依赖性的。
XN 被证明可调节衰老肝脏中的炎症、细胞凋亡和氧化应激，对肝脏改变发挥保护作用。
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