Sagan Bartłomiej, Czerny Bogusław, Stasiłowicz-Krzemień Anna, Szulc Piotr, Skomra Urszula, Karpiński Tomasz M, Lisiecka Jolanta, Kamiński Adam, Kryszak Aleksandra, Zimak-Krótkopad Oskar, Cielecka-Piontek Judyta
Department of Neurosurgery and Pediatric Neurosurgery, Pomeranian Medical University Hospital No. 1 in Szczecin, Unii Lubelskiej 1, 71-252 Szczecin, Poland.
Department of General Pharmacology and Pharmacoeconomics, Pomeranian Medical University in Szczecin, Żołnierska 48, 70-204 Szczecin, Poland.
Foods. 2024 Dec 22;13(24):4155. doi: 10.3390/foods13244155.
Hops ( L.) are widely recognized for their use in brewing, but they also possess significant pharmacological properties due to their rich bioactive compounds, with many varieties exhibiting diverse characteristics. This study investigates the chemical composition and biological activities of extracts from six hop varieties, focusing on quantifying xanthohumol and lupulone using High-Performance Liquid Chromatography (HPLC) and Total Phenolic Content (TPC) analysis. The hop varieties demonstrated significant variability in bioactive compound concentrations, with Aurora showing the highest xanthohumol (0.665 mg/g) and Zwiegniowski the highest lupulone (9.228 mg/g). TPC analysis revealed Aurora also had the highest phenolic content (22.47 mg GAE/g). Antioxidant activities were evaluated using DPPH, ABTS, CUPRAC, and FRAP assays, with Aurora and Oregon Fuggle displaying the most potent capacities. Aurora, in particular, showed the highest activity across multiple assays, including significant acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and tyrosinase inhibition, with IC values of 24.39 mg/mL, 20.38 mg/mL, and 9.37 mg/mL, respectively. The chelating activity was also assessed, with Apolon demonstrating the strongest metal ion binding capacity (IC = 1.04 mg/mL). Additionally, Aurora exhibited the most effective hyaluronidase inhibition (IC = 10.27 mg/mL), highlighting its potential for anti-inflammatory applications. The results underscore the influence of genetic and environmental factors on the bioactive compound profiles of hop varieties and their biological activity offering promising avenues for pharmaceutical and nutraceutical applications. However, further studies are needed to fully understand the potential interactions between hop cones components.
忽布（蛇麻草，Humulus lupulus L.）因其在酿造业中的应用而广为人知，但由于其富含生物活性化合物，也具有重要的药理特性，许多品种表现出不同的特征。本研究调查了六个忽布品种提取物的化学成分和生物活性，重点是使用高效液相色谱法（HPLC）和总酚含量（TPC）分析对黄腐酚和律草酮进行定量。忽布品种在生物活性化合物浓度上表现出显著差异，奥罗拉（Aurora）的黄腐酚含量最高（0.665毫克/克），兹维尼奥夫斯基（Zwiegniowski）的律草酮含量最高（9.228毫克/克）。TPC分析显示，奥罗拉的酚类含量也最高（22.47毫克没食子酸当量/克）。使用DPPH、ABTS、CUPRAC和FRAP试验评估抗氧化活性，奥罗拉和俄勒冈福格（Oregon Fuggle）表现出最强的抗氧化能力。特别是奥罗拉在多项试验中表现出最高的活性，包括对乙酰胆碱酯酶（AChE）、丁酰胆碱酯酶（BChE）和酪氨酸酶的显著抑制，IC值分别为24.39毫克/毫升、20.38毫克/毫升和9.37毫克/毫升。还评估了螯合活性，阿波罗（Apolon）表现出最强的金属离子结合能力（IC = 1.04毫克/毫升）。此外，奥罗拉表现出最有效的透明质酸酶抑制作用（IC = 10.27毫克/毫升），突出了其在抗炎应用方面的潜力。结果强调了遗传和环境因素对忽布品种生物活性化合物谱及其生物活性的影响，为制药和营养保健应用提供了有前景的途径。然而，需要进一步研究以充分了解忽布球果成分之间的潜在相互作用。
