Doyle E, Pearce J C, Picot V S, Lee R M
Smith Kline & French Research Ltd., Welwyn, Hertfordshire, U.K.
J Chromatogr. 1987 Dec 18;411:325-33. doi: 10.1016/s0021-9673(00)93983-9.
The traditional liquid-liquid extraction method for the removal of drug from biological matrix is being superseded by solid phase extraction. This involves the selection of an appropriate sorbent (normal-phase, reversed-phase, ion-exchange etc.), but once this has been achieved the method is quick and simple to operate. Most sample handling losses are avoided so recovery of drug is high and it is easily automated. Disposable columns have several advantages. Samples of 0.05-2.0 ml can be analysed routinely. Several wash stages can be included in a method to provide a specific extraction prior to a quick analysis by high-performance liquid chromatography (HPLC), radioimmunoassay, UV etc. A potential problem is that retention of the drug may involve more than one mechanism. Elution of drug may therefore require a stronger eluting solvent than analytical HPLC systems using the same stationary phase.
从生物基质中去除药物的传统液-液萃取方法正逐渐被固相萃取所取代。这涉及选择合适的吸附剂（正相、反相、离子交换等），但一旦选定，该方法操作快速且简单。大部分样品处理损失得以避免，因此药物回收率高且易于自动化操作。一次性柱有多个优点。常规可分析0.05 - 2.0毫升的样品。在高效液相色谱（HPLC）、放射免疫分析、紫外等快速分析之前，一种方法中可包含多个洗涤步骤以进行特定萃取。一个潜在问题是药物保留可能涉及不止一种机制。因此，与使用相同固定相的分析型HPLC系统相比，药物洗脱可能需要更强的洗脱溶剂。
