Azadmanesh Jahaun, Gowen Austin M, Creger Paul E, Schafer Nichole D, Blankenship Jill R
Biology Department, University of Nebraska Omaha, Nebraska 68182.
Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska 68198.
G3 (Bethesda). 2017 Nov 6;7(11):3797-3808. doi: 10.1534/g3.117.300224.
The ability of the human pathogenic fungus to switch between yeast-like and filamentous forms of growth has long been linked to pathogenesis. Numerous environmental conditions, including growth at high temperatures, nutrient limitation, and exposure to serum, can trigger this morphological switch and are frequently used in models to identify genes with roles in filamentation. Previous work has suggested that differences exist between the various models both in the genetic requirements for filamentation and transcriptional responses to distinct filamentation-inducing media, but these differences had not been analyzed in detail. We compared 10 models for filamentation and found broad genetic and transcriptomic differences between model systems. The comparative analysis enabled the discovery of novel media-independent genetic requirements for filamentation as well as a core filamentation transcriptional profile. Our data also suggest that the physical environment drives distinct programs of filamentation in , which has significant implications for filamentation .
人类致病真菌在酵母样和丝状生长形式之间转换的能力长期以来一直与发病机制相关。许多环境条件，包括在高温下生长、营养限制和暴露于血清，都可以触发这种形态转换，并且在模型中经常被用于鉴定在丝状化过程中起作用的基因。先前的研究表明，各种模型在丝状化的遗传需求以及对不同丝状化诱导培养基的转录反应方面存在差异，但这些差异尚未得到详细分析。我们比较了10种丝状化模型，发现模型系统之间存在广泛的遗传和转录组差异。这种比较分析使得能够发现丝状化新的与培养基无关的遗传需求以及核心丝状化转录谱。我们的数据还表明，物理环境在该真菌中驱动不同的丝状化程序，这对丝状化研究具有重要意义。
