Department of Physical and Analytical Chemistry, Faculty of Experimental Sciences, University of Jaén, Campus Las Lagunillas, E-23071 Jaén, Spain.
Department of Biology, Science Faculty, Selcuk University, Campus, Konya, 42130, Turkey.
Molecules. 2019 Nov 17;24(22):4171. doi: 10.3390/molecules24224171.
species are known for their use in traditional medicine. Here, we report the phenolic composition and bioactivity of methanolic and aqueous extracts of DC. leaves. The phenolic profiling and the quantitation of the main compounds were performed by high-performance liquid chromatography with diode array and mass spectrometry detections. The most abundant compounds in both extracts were caffeoylquinic acids (chlorogenic acid, particularly, with a concentration of 90.1-101.3 mg g dried extract), followed by caffeoylglucaric acids and quercetin glycosides. Antioxidant and radical scavenging assays (phosphomolybdenum, DPPH, ABTS, CUPRAC, FRAP, metal chelating activity), as well as enzyme inhibitory assays (acetylcholinesterase, butyrylcholinesterase, tyrosinase, amylase, glucosidase, and lipase), were carried out to evaluate the potential bioactivity of . The methanolic extract presented the highest antioxidant and radical scavenging values, in agreement with its higher phenolic content. Regarding enzyme inhibitory potential, the methanolic extract was also more potent than the aqueous one. Hence, leaves represent a suitable candidate for the preparation of pharmaceutical or nutraceutical products.
该物种以在传统医学中的应用而闻名。在这里，我们报告了 DC. 叶的甲醇和水提取物的酚类成分和生物活性。采用高效液相色谱-二极管阵列和质谱检测法进行酚类分析和主要化合物的定量。两种提取物中含量最丰富的化合物均为咖啡酰奎宁酸（绿原酸，特别是浓度为 90.1-101.3mg/g 干提取物），其次为咖啡酰葡萄糖酸和槲皮苷。进行了抗氧化和自由基清除测定（磷钼酸盐、DPPH、ABTS、CUPRAC、FRAP、金属螯合活性）以及酶抑制测定（乙酰胆碱酯酶、丁酰胆碱酯酶、酪氨酸酶、淀粉酶、葡萄糖苷酶和脂肪酶），以评估. 的潜在生物活性。甲醇提取物具有最高的抗氧化和自由基清除值，与其较高的酚类含量一致。关于酶抑制潜力，甲醇提取物也比水提取物更有效。因此， 叶是制备药物或营养保健品的合适候选物。
