一种用于超灵敏检测心肌肌钙蛋白 T 的玻璃纤维膜平台。
A glass fibre membrane platform for ultra-sensitive detection of cardiac troponin T.
机构信息
Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon, 200-702, Korea.
出版信息
Analyst. 2017 Oct 9;142(20):3816-3821. doi: 10.1039/c7an01389b.
A glass fibre membrane platform that allows quantification of circulating cTnT with a LoD of 0.87 pg mL is described. The proposed platform uses a glass fibre membrane, DNA-guided detection method, and antibody-conjugated fluorescent beads for the quantification of cTnT in the analytical detection range of 1-120 pg mL at room temperature in 30 min. Glass fibre membranes were chemically modified to immobilize the oligonucleotide probes that catch a biomolecular complex (FB-dAB-cTnT-cAB-DNA) containing complementary oligonucleotides. There were no interferences from human cTnI, cTnC, skTnT, biotin, and hemoglobin (each 1 μg mL). The linearity in the serial dilution test of plasma samples indicates that this platform is highly applicable for regular health check-up to assess the risk of AMI and HF.
描述了一种玻璃纤维膜平台，可实现循环 cTnT 的定量检测，检测限为 0.87pg/mL。该平台使用玻璃纤维膜、DNA 引导检测方法和抗体偶联荧光珠，可在室温下 30 分钟内定量检测分析检测范围内 1-120pg/mL 的 cTnT。玻璃纤维膜经过化学修饰，可固定寡核苷酸探针，捕获包含互补寡核苷酸的生物分子复合物（FB-dAB-cTnT-cAB-DNA）。该平台不受人 cTnI、cTnC、skTnT、生物素和血红蛋白（每种 1μg/mL）的干扰。血浆样品的系列稀释试验线性表明，该平台非常适用于常规健康检查，以评估 AMI 和 HF 的风险。
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