Kuzmin M D, Peshkova Yu I, Pashkova T M, Kartashova O L, Pashinina O A, Meshcheryakov A O
Institute of Cellular and Intracellular Symbiosis, Ural Branch of the RAS, Orenburg, Russia.
Orenburg State Medical University of Minzdrav of Russia.
Urologiia. 2017 Sep(4):18-21.
To investigate the incidence of microorganisms of different taxonomic groups and their associations in the pelvic and bladder urine of adult urolithiasis patients.
A bacteriological method and metagenomic sequencing were used to investigate the bacterial spectrum of microflora cultured from pelvic and bladder urine sampled during surgical interventions in urolithiasis patients.
The both microbiotas had approximately the same spectrum, but in 26.1% of patients it was inconsistent. Metagenomic analysis detected DNA of microorganisms in urine samples which were found free of microflora by the bacteriological method.
The study findings showed species diversity of microorganisms cultured from pelvic and bladder urine sampled during surgical interventions in urolithiasis patients.
调查成年尿路结石患者盆腔及膀胱尿液中不同分类菌群的微生物发生率及其关联。
采用细菌学方法和宏基因组测序，对尿路结石患者手术干预期间采集的盆腔及膀胱尿液中培养的微生物菌群的细菌谱进行调查。
两种微生物群的谱大致相同，但在26.1%的患者中不一致。宏基因组分析在尿液样本中检测到微生物DNA，而细菌学方法未发现这些样本中有微生物菌群。
研究结果显示了尿路结石患者手术干预期间采集的盆腔及膀胱尿液中培养的微生物的物种多样性。
